C28H36O3 420.6 1255-49-8
Action and use
Androgen.
DEFINITION
Testosterone Phenylpropionate is 3-oxo-androst-4-en-17β-yl 3-phenylpropionate. It contains not less than 97.0% and not more than 103.0% of C28H36O3, calculated with reference to the dried substance.
CHARACTERISTICS
A white to almost white, crystalline powder.
Practically insoluble in water; sparingly soluble in ethanol (96%).
IDENTIFICATION
A. The infrared absorption spectrum, Appendix II A, is concordant with the reference spectrum of testosterone phenylpropionate (RSV 43). If the spectra are not concordant, dissolve the substances in the minimum volume of dichloromethane, evaporate to dryness and prepare a new spectrum of the residue.
B. Complies with the test for identification of steroids, Appendix III A, using impregnating solvent III and mobile phase F.
C. Dissolve 25 mg in 1 mL of methanol, add 2 mL of semicarbazide acetate solution, heat under a reflux condenser for 30 minutes and cool. The melting point of the resulting precipitate is about 218°, Appendix V A.
TESTS
Melting point
114° to 117°, Appendix V A.
Specific optical rotation
In a 1% w/v solution in 1,4-dioxan, +86 to +91, calculated with reference to the dried substance, Appendix V F.
Loss on drying
When dried to constant weight at 105°, loses not more than 0.5% of its weight. Use 1 g.
Sulfated ash
Not more than 0.1%, Appendix IX A.
ASSAY
Dissolve 10 mg in sufficient absolute ethanol to produce 100 mL, dilute 5 mL to 50 mL with absolute ethanol and measure the absorbance of the resulting solution at the maximum at 240 nm, Appendix II B. Calculate the content of C28H36O3 taking 395 as the value of A(1%, 1 cm) at the maximum at 240 nm.
STORAGE
Testosterone Phenylpropionate should be protected from light.
