{"id":960,"date":"2025-09-17T10:18:28","date_gmt":"2025-09-17T03:18:28","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=960"},"modified":"2025-10-03T11:44:22","modified_gmt":"2025-10-03T04:44:22","slug":"edrophonium-chloride","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/edrophonium-chloride\/","title":{"rendered":"Edrophonium Chloride"},"content":{"rendered":"<p><em>(Ph. Eur. monograph 2106)<\/em><\/p>\n<p>C<sub>10<\/sub>H<sub>16<\/sub>ClNO 201.7 116-38-1<\/p>\n<p><strong>Action and use<\/strong><\/p>\n<p>Cholinesterase inhibitor.<\/p>\n<p><strong>Preparation<\/strong><\/p>\n<p>Edrophonium Injection<\/p>\n<h2>DEFINITION<\/h2>\n<p>N-Ethyl-3-hydroxy-N,N-dimethylanilinium chloride.<\/p>\n<h3>Content<\/h3>\n<p>99.0 per cent to 101.0 per cent (dried substance).<\/p>\n<h2>CHARACTERS<\/h2>\n<h3>Appearance<\/h3>\n<p>White or almost white, crystalline powder.<\/p>\n<h3>Solubility<\/h3>\n<p>Very soluble in water, freely soluble in ethanol (96 per cent), practically insoluble in methylene chloride.<\/p>\n<h2>IDENTIFICATION<\/h2>\n<p>A. Infrared absorption spectrophotometry (2.2.24).<\/p>\n<p><em>Comparison<\/em>\u00a0 edrophonium chloride CRS.<\/p>\n<p>B. It gives reaction (a) of chlorides (2.3.1).<\/p>\n<h2>TESTS<\/h2>\n<h3>Appearance of solution<\/h3>\n<p>The solution is clear (2.2.1) and colourless (2.2.2, Method II).<\/p>\n<p>Dissolve 0.5 g in water R and dilute to 25 mL with the same solvent.<\/p>\n<h3>pH (2.2.3)<\/h3>\n<p>4.0 to 5.0.<\/p>\n<p>Dissolve 1.0 g in carbon dioxide-free water R and dilute to 10.0 mL with the same solvent.<\/p>\n<h3>Related substances<\/h3>\n<p>Liquid chromatography (2.2.29).<\/p>\n<p><em>Test solution<\/em>\u00a0 Dissolve 50.0 mg in water R and dilute to 50.0 mL with the same solvent.<\/p>\n<p><em>Reference solution (a)<\/em>\u00a0 Dissolve 10.0 mg of 3-dimethylaminophenol R in acetonitrile R and dilute to 10.0 mL with the same solvent.<\/p>\n<p><em>Reference solution (b)<\/em>\u00a0 Mix 1.0 mL of the test solution and 1.0 mL of reference solution (a) and dilute to 100.0 mL with water R. Dilute 10.0 mL of this solution to 100.0 mL with water R.<\/p>\n<p><em>Column:<\/em><\/p>\n<p><em>\u2014 size:<\/em> l = 0.25 m, \u00d8 = 4.6 mm,<\/p>\n<p><em>\u2014 stationary phase:<\/em> styrene-divinylbenzene copolymer R (8-10 \u03bcm).<\/p>\n<p><em>Mobile phase<\/em>\u00a0 Mix 10 volumes of acetonitrile R and 90 volumes of a 7.7 g\/L solution of tetramethylammonium bromide R previously adjusted to pH 3.0 with phosphoric acid R.<\/p>\n<p><em>Flow rate<\/em>\u00a0 1 mL\/min.<\/p>\n<p><em>Detection<\/em>\u00a0 Spectrophotometer at 281 nm.<\/p>\n<p><em>Injection<\/em>\u00a0 20 \u03bcL.<\/p>\n<p><em>Run time<\/em> Twice the retention time of edrophonium.<\/p>\n<p><em>Relative retention<\/em>\u00a0 With reference to edrophonium (retention time = about 3.8 min): impurity A = about 1.3.<\/p>\n<p><em>System suitability<\/em>\u00a0 Reference solution (b):<\/p>\n<p><em>\u2014 resolution:<\/em> minimum 2.0 between the peaks due to edrophonium and impurity A.<\/p>\n<p><em>Limits:<\/em><\/p>\n<p><em>\u2014 impurity A:<\/em> not more than the area of the corresponding peak in the chromatogram obtained with reference<br \/>\nsolution (b) (0.1 per cent),<\/p>\n<p><em>\u2014 any other impurity:<\/em> for each impurity, not more than the area of the peak due to edrophonium in the chromatogram obtained with reference solution (b) (0.1 per cent),<\/p>\n<p><em>\u2014 total:<\/em> not more than 5 times the area of the peak due to edrophonium in the chromatogram obtained with reference solution (b) (0.5 per cent),<\/p>\n<p><em>\u2014 disregard limit:<\/em> 0.5 times the area of the peak due to edrophonium in the chromatogram obtained with reference solution (b) (0.05 per cent).<\/p>\n<h3>Loss on drying (2.2.32)<\/h3>\n<p>Maximum 0.5 per cent, determined on 1.000 g by drying in a desiccator at a pressure not exceeding 0.7 kPa for 24 h.<\/p>\n<h3>Sulfated ash (2.4.14)<\/h3>\n<p>Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n<h3>Bacterial endotoxins (2.6.14)<\/h3>\n<p>Less than 8.3 IU\/mg.<\/p>\n<h2>ASSAY<\/h2>\n<p>Dissolve 0.150 g in 60 mL of a mixture of equal volumes of acetic anhydride R and anhydrous acetic acid R. Titrate with 0.1 M perchloric acid, determining the end-point potentiometrically (2.2.20).<\/p>\n<p>1 mL of 0.1 M perchloric acid is equivalent to 20.17 mg of C<sub>10<\/sub>H<sub>16<\/sub>ClNO.<\/p>\n<h2>STORAGE<\/h2>\n<p>Protected from light.<\/p>\n<h2>IMPURITIES<\/h2>\n<p>Specified impurities A.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-8486\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/Edrophonium-Chloride-300x163.jpg\" alt=\"Edrophonium Chloride\" width=\"300\" height=\"163\" srcset=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/Edrophonium-Chloride-300x163.jpg 300w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/Edrophonium-Chloride-1024x555.jpg 1024w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/Edrophonium-Chloride-768x416.jpg 768w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/Edrophonium-Chloride.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>A. 3-(dimethylamino)phenol.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>(Ph. Eur. monograph 2106) C10H16ClNO 201.7 116-38-1 Action and use Cholinesterase inhibitor. Preparation Edrophonium Injection DEFINITION N-Ethyl-3-hydroxy-N,N-dimethylanilinium chloride. Content 99.0 per cent to 101.0 per cent (dried substance). CHARACTERS Appearance White or almost white, crystalline powder. Solubility Very soluble in water, freely soluble in ethanol (96 per cent), practically insoluble in methylene chloride. IDENTIFICATION A&#8230;.<\/p>\n","protected":false},"author":1,"featured_media":995,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-960","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/960","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=960"}],"version-history":[{"count":8,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/960\/revisions"}],"predecessor-version":[{"id":8487,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/960\/revisions\/8487"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/995"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=960"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=960"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=960"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}