{"id":5315,"date":"2025-09-29T11:34:27","date_gmt":"2025-09-29T04:34:27","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=5315"},"modified":"2025-10-03T09:27:27","modified_gmt":"2025-10-03T02:27:27","slug":"calcium-glucoheptonate","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/calcium-glucoheptonate\/","title":{"rendered":"Calcium Glucoheptonate"},"content":{"rendered":"<p>(Ph. Eur. monograph 1399)<\/p>\n<p>C<sub>14<\/sub>H<sub>26<\/sub>CaO<sub>16<\/sub>\u00a0 \u00a0490.4<\/p>\n<p><strong>Action and use<\/strong><\/p>\n<p>Used in treatment of calcium deficiency.<\/p>\n<h2>DEFINITION<\/h2>\n<p>Mixture in variable proportions, of calcium di(D-glycero-D-gulo-heptonate) and calcium di(D-glycero-D-ido-heptonate).<\/p>\n<h3>Content<\/h3>\n<p>98.0 per cent to 102.0 per cent of calcium 2,3,4,5,6,7-hexahydroxyheptanoate (dried substance).<\/p>\n<h2>CHARACTERS<\/h2>\n<h3>Appearance<\/h3>\n<p>White or very slightly yellow, amorphous powder, hygroscopic.<\/p>\n<h3>Solubility<\/h3>\n<p>Very soluble in water, practically insoluble in acetone and in ethanol (96 per cent).<\/p>\n<h2>IDENTIFICATION<\/h2>\n<p>A. Thin-layer chromatography (2.2.27).<\/p>\n<p>Test solution: Dissolve 20 mg of the substance to be examined in 1 mL of water R.<\/p>\n<p>Reference solution (a): Dissolve 20 mg of calcium glucoheptonate CRS in 1 mL of water R.<\/p>\n<p>Reference solution (b): Dissolve 10 mg of calcium gluconate CRS in 0.5 mL of the test solution and dilute to 1 mL with water R.<\/p>\n<p>Plate: cellulose for chromatography R1 as the coating substance.<\/p>\n<p>Mobile phase: formic acid R, water R, acetone R, butanol R (20:20:30:30 V\/V\/V\/V); use a freshly prepared mixture.<\/p>\n<p>Application: 10 \u03bcL as bands of 20 mm by 2 mm.<\/p>\n<p>Development: In a tank previously allowed to saturate for 10 min, over a path of 12 cm.<\/p>\n<p>Drying: In air.<\/p>\n<p>Detection: Spray with 0.02 M potassium permanganate.<\/p>\n<p>System suitability: Reference solution (b):<\/p>\n<p>\u2014 the chromatogram shows 2 clearly separated spots.<\/p>\n<p>Results: The principal spot in the chromatogram obtained with the test solution is similar in position and size to the principal spot in the chromatogram obtained with reference solution (a).<\/p>\n<p>B. 0.2 mL of solution S (see Tests) gives reaction (b) of calcium (2.3.1).<\/p>\n<h2>TESTS<\/h2>\n<h3>Solution S<\/h3>\n<p>Dissolve 10.0 g in carbon dioxide-free water R prepared from distilled water R and dilute to 100 mL with the same solvent.<\/p>\n<h3>Appearance of solution<\/h3>\n<p>Solution S is clear (2.2.1) and not more intensely coloured than reference solution Y6 (2.2.2, Method II).<\/p>\n<h3>pH (2.2.3)<\/h3>\n<p>6.0 to 8.0 for solution S.<\/p>\n<h3>Reducing sugars<\/h3>\n<p>Maximum 1 per cent, expressed as glucose.<\/p>\n<p>Dissolve 1.0 g in 5 mL of water R with the aid of gentle heat. Cool and add 20 mL of cupri-citric solution R and a few glass beads. Heat so that boiling begins after 4 min and maintain boiling for 3 min. Cool rapidly and add 100 mL of a 2.4 per cent V\/V solution of glacial acetic acid R and 20.0 mL of 0.025 M iodine. With continuous shaking, add 25 mL of a mixture<br \/>\nof 6 volumes of hydrochloric acid R and 94 volumes of water R until the precipitate dissolves, titrate the excess of iodine with 0.05 M sodium thiosulfate using 1 mL of starch solution R added towards the end of the titration, as indicator. Not less than 12.6 mL of 0.05 M sodium thiosulfate is required.<\/p>\n<h3>Cyanide<\/h3>\n<p>Dissolve 5.0 g in 50 mL of water R and add 2.0 g of tartaric acid R. Place this solution in a distillation apparatus (2.2.11).<\/p>\n<p>The plain bend adapter attached to the end of the condenser has a vertical part that is long enough to extend to 1 cm from the bottom of a 50 mL test-tube used as a receiver. Place 10 mL of water R and 2 mL of 0.1 M sodium hydroxide into the receiver. Distil, collect 25 mL of distillate and dilute to 50 mL with water R. To 25 mL of this solution add 25 mg of ferrous<br \/>\nsulfate R and boil for a short time. After cooling to about 70 \u00b0C add 10 mL of hydrochloric acid R1. After 30 min, filter the solution and wash the filter. A yellow spot appears on the filter; there is no blue or green spot.<\/p>\n<h3>Chlorides (2.4.4)<\/h3>\n<p>Maximum 100 ppm.<\/p>\n<p>To 5 mL of solution S, add 10 mL of water R.<\/p>\n<h3>Sulfates (2.4.13)<\/h3>\n<p>Maximum 100 ppm, determined on solution S.<\/p>\n<h3>Iron (2.4.9)<\/h3>\n<p>Maximum 40 ppm.<\/p>\n<p>Dilute 2.5 mL of solution S to 10 mL with water R.<\/p>\n<h3>Loss on drying (2.2.32)<\/h3>\n<p>Maximum 5.0 per cent, determined on 1.000 g by drying in an oven at 105 \u00b0C for 3 h.<\/p>\n<h3>Bacterial endotoxins (2.6.14)<\/h3>\n<p>Less than 167 IU\/g, if intended for use in the manufacture of parenteral preparations without a further appropriate procedure for the removal of bacterial endotoxins.<\/p>\n<h2>ASSAY<\/h2>\n<p>Dissolve 0.800 g in a mixture of 2 mL of 3 M hydrochloric acid R and 150 mL of water R. While stirring, add 12.5 mL of<\/p>\n<p>0.1 M sodium edetate, 15 mL of 1 M sodium hydroxide and 0.3 g of hydroxynaphthol blue, sodium salt R. Titrate with 0.1 M sodium edetate until the colour changes from violet to pure blue.<\/p>\n<p>1 mL of 0.1 M sodium edetate is equivalent to 49.04 mg of C<sub>14<\/sub>H<sub>26<\/sub>CaO<sub>16<\/sub>.<\/p>\n<h2>STORAGE<\/h2>\n<p>In an airtight container. If the substance is sterile, store in a sterile, airtight, tamper-evident container.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>(Ph. Eur. monograph 1399) C14H26CaO16\u00a0 \u00a0490.4 Action and use Used in treatment of calcium deficiency. DEFINITION Mixture in variable proportions, of calcium di(D-glycero-D-gulo-heptonate) and calcium di(D-glycero-D-ido-heptonate). Content 98.0 per cent to 102.0 per cent of calcium 2,3,4,5,6,7-hexahydroxyheptanoate (dried substance). CHARACTERS Appearance White or very slightly yellow, amorphous powder, hygroscopic. Solubility Very soluble in water, practically&#8230;<\/p>\n","protected":false},"author":4,"featured_media":5337,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-5315","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/5315","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=5315"}],"version-history":[{"count":3,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/5315\/revisions"}],"predecessor-version":[{"id":5886,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/5315\/revisions\/5886"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/5337"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=5315"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=5315"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=5315"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}