{"id":4696,"date":"2025-09-27T11:22:38","date_gmt":"2025-09-27T04:22:38","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=4696"},"modified":"2025-10-02T15:58:55","modified_gmt":"2025-10-02T08:58:55","slug":"carbachol","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/carbachol\/","title":{"rendered":"Carbachol"},"content":{"rendered":"

(Ph. Eur. monograph 1971)<\/p>\n

C6<\/sub>H15<\/sub>ClN2<\/sub>O2<\/sub>\u00a0 \u00a0 \u00a0 182.7\u00a0 \u00a0 \u00a0 51-83-2<\/p>\n

Action and use<\/strong><\/p>\n

Cholinoceptor agonist.<\/p>\n

DEFINITION<\/h2>\n

2-(Carbamoyloxy)-N,N,N-trimethylethanaminium chloride.<\/p>\n

Content<\/h3>\n

99.0 per cent to 101.5 per cent (dried substance).<\/p>\n

CHARACTERS<\/h2>\n

Appearance<\/h3>\n

White or almost white, crystalline, hygroscopic powder.<\/p>\n

Solubility<\/h3>\n

Very soluble in water, sparingly soluble in ethanol (96 per cent), practically insoluble in acetone.<\/p>\n

IDENTIFICATION<\/h2>\n

First identification: A, C.<\/p>\n

Second identification: B, C.<\/p>\n

A. Infrared absorption spectrophotometry (2.2.24).<\/p>\n

Comparison: carbachol CRS.<\/p>\n

B. Examine the chromatograms obtained in the test for related substances.<\/p>\n

Results: The principal spot in the chromatogram obtained with test solution (b) is similar in position, colour and size to the principal spot in the chromatogram obtained with reference solution (a).<\/p>\n

C. 0.5 mL of solution S (see Tests) gives reaction (a) of chlorides (2.3.1).<\/p>\n

TESTS<\/h2>\n

Solution S<\/h3>\n

Dissolve 2.5 g in carbon dioxide-free water R and dilute to 25 mL with the same solvent.<\/p>\n

Appearance of solution<\/h3>\n

Solution S is clear (2.2.1) and colourless (2.2.2, Method II).<\/p>\n

Acidity or alkalinity<\/h3>\n

To 2.0 mL of solution S, add 0.05 mL of methyl red mixed solution R. Not more than 0.2 mL of 0.01 M hydrochloric acid or 0.01 M sodium hydroxide is required to change the colour of the indicator.<\/p>\n

Related substances<\/h3>\n

Thin-layer chromatography (2.2.27).<\/p>\n

Prepare the solutions immediately before use.<\/p>\n

Test solution (a): Dissolve 0.20 g of the substance to be examined in methanol R and dilute to 5.0 mL with the same solvent.<\/p>\n

Test solution (b): Dilute 2.0 mL of test solution (a) to 20.0 mL with methanol R.<\/p>\n

Reference solution (a): Dissolve 20 mg of carbachol CRS in methanol R and dilute to 5.0 mL with the same solvent.<\/p>\n

Reference solution (b): Dissolve 8 mg of choline chloride R and 8 mg of acetylcholine chloride CRS in methanol R and dilute to 10.0 mL with the same solvent. Dilute 5.0 mL to 10.0 mL with methanol R.<\/p>\n

Plate: cellulose for chromatography R as the coating substance.<\/p>\n

Mobile phase: water R, methanol R (10:90 V\/V).<\/p>\n

Application: 10 \u03bcL.<\/p>\n

Development: Over 2\/3 of the plate.<\/p>\n

Detection: Spray with potassium iodobismuthate solution R3.<\/p>\n

System suitability: The chromatogram obtained with reference solution (b) shows 2 clearly separated spots.<\/p>\n

Limits: In the chromatogram obtained with test solution (a):<\/p>\n

\u2014 any impurity: any spot, apart from the principal spot, is not more intense than one or other of the 2 principal spots in the chromatogram obtained with reference solution (b) (1 per cent). Compare the spots with the spot of the most appropriate colour in the chromatogram obtained with reference solution (b).<\/p>\n

Loss on drying (2.2.32)<\/h4>\n

Maximum 1.0 per cent, determined on 1.000 g by drying in an oven at 105 \u00b0C for 2 h.<\/p>\n

Sulfated ash (2.4.14)<\/h4>\n

Maximum 0.1 per cent, determined on 1.0 g of the residue obtained in the test for loss on drying.<\/p>\n

ASSAY<\/h3>\n

Dissolve 0.150 g in a mixture of 10 mL of anhydrous acetic acid R and 40 mL of acetic anhydride R. Titrate with 0.1 M perchloric acid. Determine the end-point potentiometrically (2.2.20).<\/p>\n

1 mL of 0.1 M perchloric acid is equivalent to 18.27 mg of C6<\/sub>H15<\/sub>ClN2<\/sub>O2<\/sub>.<\/p>\n

STORAGE<\/h2>\n

In an airtight container, protected from light.<\/p>\n

IMPURITIES<\/h2>\n

\"Carbachol\"<\/p>\n

A. 2-hydroxy-N,N,N-trimethylethanaminium chloride (choline chloride).<\/p>\n","protected":false},"excerpt":{"rendered":"

(Ph. Eur. monograph 1971) C6H15ClN2O2\u00a0 \u00a0 \u00a0 182.7\u00a0 \u00a0 \u00a0 51-83-2 Action and use Cholinoceptor agonist. DEFINITION 2-(Carbamoyloxy)-N,N,N-trimethylethanaminium chloride. Content 99.0 per cent to 101.5 per cent (dried substance). CHARACTERS Appearance White or almost white, crystalline, hygroscopic powder. Solubility Very soluble in water, sparingly soluble in ethanol (96 per cent), practically insoluble in acetone. IDENTIFICATION…<\/p>\n","protected":false},"author":2,"featured_media":4719,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-4696","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/4696","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=4696"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/4696\/revisions"}],"predecessor-version":[{"id":5327,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/4696\/revisions\/5327"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/4719"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=4696"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=4696"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=4696"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}