{"id":3491,"date":"2025-09-24T13:49:08","date_gmt":"2025-09-24T06:49:08","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=3491"},"modified":"2025-11-15T12:55:21","modified_gmt":"2025-11-15T05:55:21","slug":"benzalkonium-chloride-solution","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/benzalkonium-chloride-solution\/","title":{"rendered":"Benzalkonium Chloride Solution"},"content":{"rendered":"

(Ph. Eur. monograph 0371)<\/p>\n

Action and use<\/strong><\/p>\n

Antiseptic.<\/p>\n

DEFINITION<\/h2>\n
Aqueous solution of a mixture of alkylbenzyldimethylammonium chlorides, the alkyl groups mainly having chain lengths of C_{12<\/sub>, C_{14<\/sub> and C_{16<\/sub>.<\/p>\n}}}
Content<\/h3>\n
475 g\/L to 525 g\/L of alkylbenzyldimethylammonium chlorides, calculated using the average relative molecular mass (see Tests). The solution may contain ethanol (96 per cent).<\/p>\n
CHARACTERS<\/h2>\n
Appearance<\/h3>\n
Clear, colourless or slightly yellowish liquid.<\/p>\n
Solubility<\/h3>\n
Miscible with water and with ethanol (96 per cent).<\/p>\n
It froths copiously when shaken.<\/p>\n
IDENTIFICATION<\/h2>\n
First identification: B, E.<\/p>\n
Second identification: A, C, D, E.<\/p>\n
A. Ultraviolet and visible absorption spectrophotometry (2.2.25).<\/p>\n
Test solution: Dilute 0.3 mL to 100.0 mL with water R.<\/p>\n
Spectral range: 220-350 nm.<\/p>\n
Absorption maxima: At 257 nm, 263 nm and 269 nm.<\/p>\n
Shoulder: At about 250 nm.<\/p>\n
B. Examine the chromatograms obtained in the test for average relative molecular mass and ratio of alkyl components.<\/p>\n
Results: The principal peaks in the chromatogram obtained with the test solution are similar in retention time to the principal peaks in the chromatogram obtained with the reference solution.<\/p>\n
C. To 0.05 mL add 2 mL of water R, 0.1 mL of glacial acetic acid R and, dropwise, 1 mL of sodium tetraphenylborate solution R. A white precipitate is formed. Filter. Dissolve the precipitate in a mixture of 1 mL of acetone R and 5 mL of ethanol (96 per cent) R, heating to not more than 70 \u00b0C. Add water R dropwise to the warm solution until a slight opalescence forms. Heat gently until the solution is clear and allow to cool. White crystals separate. Filter, wash with 3 quantities, each of 10 mL, of water R and dry in vacuo (2.2.32) at a temperature not exceeding 50 \u00b0C. The crystals melt (2.2.14) at 127 \u00b0C to 133 \u00b0C.<\/p>\n
D. To 5 mL of dilute sodium hydroxide solution R add 0.1 mL of bromophenol blue solution R1 and 5 mL of methylene chloride R and shake. The methylene chloride layer is colourless. Add 0.05 mL of the solution to be examined and shake. The methylene chloride layer becomes blue.<\/p>\n
E. To 0.05 mL add 1 mL of dilute nitric acid R. A white precipitate is formed which dissolves on the addition of 5 mL of ethanol (96 per cent) R. The solution gives reaction (a) of chlorides (2.3.1).<\/p>\n
TESTS<\/h2>\n
Solution S<\/h3>\n
Dilute 2.0 g to 100 mL with carbon dioxide-free water R.<\/p>\n
Appearance of solution<\/h3>\n
Solution S is clear (2.2.1) and not more intensely coloured than reference solution Y_{6<\/sub> (2.2.2, Method II).<\/p>\n}
Acidity or alkalinity<\/h3>\n
To 50 mL of solution S add 0.1 mL of bromocresol purple solution R. Not more than 0.1 mL of 0.1 M hydrochloric acid or 0.1 M sodium hydroxide is required to change the colour of the indicator.<\/p>\n
Average relative molecular mass and ratio of alkyl components<\/h3>\n
Liquid chromatography (2.2.29).<\/p>\n
Test solution: Determine the density (2.2.5) of the solution to be examined. Dilute a quantity of the solution to be examined equivalent to about 0.400 g of benzalkonium chloride to 100.0 mL with water R.<\/p>\n
Reference solution: Dissolve the contents of a vial of benzalkonium chloride for system suitability CRS in 5 mL of water R.<\/p>\n
Column:<\/p>\n
\u2014 size: l = 0.25 m, \u00d8 = 4.6 mm;<\/p>\n
\u2014 stationary phase: end-capped cyanosilyl silica gel for chromatography R (5 \u03bcm).<\/p>\n
Mobile phase: Mix 45 volumes of acetonitrile R and 55 volumes of a 13.6 g\/L solution of sodium acetate R previously adjusted to pH 5.0 with glacial acetic acid R.<\/p>\n
Flow rate: 2.0 mL\/min.<\/p>\n
Detection: Spectrophotometer at 254 nm.<\/p>\n
Injection: 10 \u03bcL.<\/p>\n
Identification of homologues: Use the chromatogram supplied with benzalkonium chloride for system suitability CRS and the chromatogram obtained with the reference solution to identify the peaks due to homologues C_{12<\/sub>, C_{14<\/sub> and C_{16<\/sub>.<\/p>\n}}}
Relative retention: With reference to C_{12<\/sub> homologue (retention time = about 6 min): C_{14<\/sub> homologue = about 1.3; C_{16<\/sub> homologue = about 1.7.<\/p>\n}}}
System suitability: Reference solution:<\/p>\n
\u2014 resolution: minimum 1.5 between the peaks due to the C12 and C14 homologues.<\/p>\n
Calculate the average relative molecular mass of the sample by summing the products for each homologue, using the following expression:<\/p>\n
$\"Benzalkonium$ <\/p>\n
A = area of the peak due to the given homologue in the chromatogram obtained with the test solution;<\/p>\n
B = sum of the areas of the peaks due to all homologues in the chromatogram obtained with the test solution;<\/p>\n
W = relative molecular mass for the given homologue: 340, 368 and 396 for the C_{12<\/sub>, C_{14<\/sub> and C_{16<\/sub> homologues, respectively.<\/p>\n}}}
Calculate the percentage of each homologue, using the following expression:<\/p>\n
$\"Benzalkonium$ <\/p>\n
C = product of the relative molecular mass of the given homologue and the area of the corresponding peak in the chromatogram obtained with the test solution;<\/p>\n
D = sum of the C values for all homologues quantified.<\/p>\n
Limits:<\/p>\n
\u2014 C_{12<\/sub> homologue: minimum 40 per cent;<\/p>\n}
\u2014 C_{14<\/sub> homologue: minimum 20 per cent;<\/p>\n}
\u2014 sum of C_{12 <\/sub>and C_{14<\/sub> homologues: minimum 70 per cent.<\/p>\n}}
Impurities A, B and C<\/h3>\n
Liquid chromatography (2.2.29). Prepare the solutions immediately before use.<\/p>\n
Test solution Determine the density (2.2.5) of the solution to be examined. Dilute a quantity of the solution to be examined equivalent to 2.5 g of benzalkonium chloride to 50.0 mL with methanol R.<\/p>\n
Reference solution (a): Dissolve 25.0 mg of benzyl alcohol CRS (impurity A) in methanol R and dilute to 100.0 mL with the same solvent.<\/p>\n
Reference solution (b): Dissolve 75.0 mg of benzaldehyde CRS (impurity B) in methanol R and dilute to 100.0 mL with the same solvent. Dilute 1.0 mL of this solution to 10.0 mL with methanol R.<\/p>\n
Reference solution (c:) Dilute 1 mL of reference solution (a) to 10 mL with methanol R.<\/p>\n
Column:<\/p>\n
\u2014 size: l = 0.15 m, \u00d8 = 4.6 mm;<\/p>\n
\u2014 stationary phase: end-capped octadecylsilyl silica gel for chromatography R (5 \u03bcm);<\/p>\n
\u2014 temperature: 30 \u00b0C.<\/p>\n
Mobile phase:<\/p>\n
\u2014 mobile phase A: dissolve 1.09 g of sodium hexanesulfonate R and 6.9 g of sodium dihydrogen phosphate monohydrate R in water for chromatography R; adjust to pH 3.5 with phosphoric acid R and dilute to 1000 mL with the same solvent;<\/p>\n
\u2014 mobile phase B: methanol R2;<\/p>\n\n\n\n\n\n\n\n\n
Time<\/strong>
\n(min)<\/strong><\/td>\n Mobile phase A<\/strong>
\n(per cent V\/V)<\/strong><\/td>\n Mobile phase B<\/strong>
\n(per cent V\/V)<\/strong><\/td>\n<\/tr>\n
0 – 10<\/td>\n 80<\/td>\n 20<\/td>\n<\/tr>\n
10 – 14<\/td>\n 80 \u2192 50<\/td>\n 20 \u2192 50<\/td>\n<\/tr>\n
14 – 35<\/td>\n 50<\/td>\n 50<\/td>\n<\/tr>\n
35 – 36<\/td>\n \u00a050 \u2192 20<\/td>\n 50 \u2192 80<\/td>\n<\/tr>\n
36 – 55<\/td>\n 20<\/td>\n 80<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
Flow rate:1.0 mL\/min.<\/p>\n
Detection: Spectrophotometer at 210 nm for impurities A and C, and at 257 nm for impurity B.<\/p>\n
Injection: 20 \u03bcL.<\/p>\n
Relative retention: With reference to impurity A (retention time = about 10 min): impurity B = about 1.3; impurity C = about 2.4.<\/p>\n
System suitability: At 210 nm:<\/p>\n
\u2014 signal-to-noise ratio: minimum 10 for the principal peak in the chromatogram obtained with reference solution (c);<\/p>\n
\u2014 symmetry factor: minimum 0.6 for the peak due to impurity A in the chromatogram obtained with reference solution (a).<\/p>\n
Limits:<\/p>\n
\u2014 correction factor: for the calculation of content, multiply the peak area of impurity C by 1.3;<\/p>\n
\u2014 impurity A: not more than the area of the corresponding peak in the chromatogram obtained with reference solution (a) (0.5 per cent);<\/p>\n
\u2014 impurity B: not more than the area of the corresponding peak in the chromatogram obtained with reference solution (b) (0.15 per cent);<\/p>\n
\u2014 impurity C: not more than 0.1 times the area of the principal peak in the chromatogram obtained with reference solution (a) (0.05 per cent).<\/p>\n
Amines and amine salts<\/h3>\n
Mix 10.0 g, while heating, with 20 mL of a mixture of 3 volumes of 1 M hydrochloric acid and 97 volumes of methanol R and add 100 mL of 2-propanol R. Pass a stream of nitrogen R slowly through the solution. Titrate with up to 12.0 mL of 0.1 M tetrabutylammonium hydroxide and record the potentiometric titration curve (2.2.20). If the curve shows 2 points of inflexion, the volume of titrant added between the 2 points is not greater than 5.0 mL. If the curve shows no point of inflexion, the solution to be examined does not comply with the test. If the curve shows 1 point of inflexion, repeat the test but add 3.0 mL of a 25.0 g\/L solution of dimethyldecylamine R in 2-propanol R before the titration. If the titration curve after the addition of 12.0 mL of the titrant shows only 1 point of inflexion, the solution to be examined does not comply with the test.<\/p>\n
Sulfated ash (2.4.14)<\/h4>\n
Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n
ASSAY<\/h2>\n
Determine the density (2.2.5) of the solution to be examined. Dilute 4.00 g to 100.0 mL with water R. Transfer 25.0 mL of the solution to a separating funnel, add 25 mL of methylene chloride R, 10 mL of 0.1 M sodium hydroxide and 10.0 mL of a freshly prepared 50 g\/L solution of potassium iodide R. Shake well, allow to separate and discard the methylene chloride layer. Shake the aqueous layer with 3 quantities, each of 10 mL, of methylene chloride R and discard the methylene chloride layers. To the aqueous layer add 40 mL of hydrochloric acid R, allow to cool and titrate with 0.05 M potassium iodate until the deep-brown colour is almost discharged. Add 5 mL of methylene chloride R and continue the titration, shaking vigorously, until the methylene chloride layer no longer changes colour. Carry out a blank titration on a mixture of 10.0 mL of the freshly prepared 50 g\/L solution of potassium iodide R, 20 mL of water R and 40 mL of hydrochloric acid R.<\/p>\n
1 mL of 0.05 M potassium iodate is equivalent to mg of benzalkonium chloride where x is the average relative molecular mass of the sample.<\/p>\n
LABELLING<\/h2>\n
The label states the content of ethanol (96 per cent), if any.<\/p>\n
IMPURITIES<\/h2>\n
Specified impurities A, B, C.<\/p>\n
$\"Benzalkonium$ <\/p>\n
A. benzyl alcohol,<\/p>\n
$\"Benzalkonium$ <\/p>\n
B. benzaldehyde,<\/p>\n
$\"Benzalkonium$ <\/p>\n
C. (chloromethyl)benzene.<\/p>\n","protected":false},"excerpt":{"rendered":"
(Ph. Eur. monograph 0371) Action and use Antiseptic. DEFINITION Aqueous solution of a mixture of alkylbenzyldimethylammonium chlorides, the alkyl groups mainly having chain lengths of C12, C14 and C16. Content 475 g\/L to 525 g\/L of alkylbenzyldimethylammonium chlorides, calculated using the average relative molecular mass (see Tests). The solution may contain ethanol (96 per cent)….<\/p>\n","protected":false},"author":2,"featured_media":3529,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-3491","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/3491","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=3491"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/3491\/revisions"}],"predecessor-version":[{"id":5227,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/3491\/revisions\/5227"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/3529"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=3491"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=3491"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=3491"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

Time<\/strong> \n(min)<\/strong><\/td>\n	Mobile phase A<\/strong> \n(per cent V\/V)<\/strong><\/td>\n	Mobile phase B<\/strong> \n(per cent V\/V)<\/strong><\/td>\n<\/tr>\n
0 – 10<\/td>\n	80<\/td>\n	20<\/td>\n<\/tr>\n
10 – 14<\/td>\n	80 \u2192 50<\/td>\n	20 \u2192 50<\/td>\n<\/tr>\n
14 – 35<\/td>\n	50<\/td>\n	50<\/td>\n<\/tr>\n
35 – 36<\/td>\n	\u00a050 \u2192 20<\/td>\n	50 \u2192 80<\/td>\n<\/tr>\n
36 – 55<\/td>\n	20<\/td>\n	80<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Flow rate:1.0 mL\/min.<\/p>\n Detection: Spectrophotometer at 210 nm for impurities A and C, and at 257 nm for impurity B.<\/p>\n Injection: 20 \u03bcL.<\/p>\n Relative retention: With reference to impurity A (retention time = about 10 min): impurity B = about 1.3; impurity C = about 2.4.<\/p>\n System suitability: At 210 nm:<\/p>\n \u2014 signal-to-noise ratio: minimum 10 for the principal peak in the chromatogram obtained with reference solution (c);<\/p>\n \u2014 symmetry factor: minimum 0.6 for the peak due to impurity A in the chromatogram obtained with reference solution (a).<\/p>\n Limits:<\/p>\n \u2014 correction factor: for the calculation of content, multiply the peak area of impurity C by 1.3;<\/p>\n \u2014 impurity A: not more than the area of the corresponding peak in the chromatogram obtained with reference solution (a) (0.5 per cent);<\/p>\n \u2014 impurity B: not more than the area of the corresponding peak in the chromatogram obtained with reference solution (b) (0.15 per cent);<\/p>\n \u2014 impurity C: not more than 0.1 times the area of the principal peak in the chromatogram obtained with reference solution (a) (0.05 per cent).<\/p>\n Amines and amine salts<\/h3>\n Mix 10.0 g, while heating, with 20 mL of a mixture of 3 volumes of 1 M hydrochloric acid and 97 volumes of methanol R and add 100 mL of 2-propanol R. Pass a stream of nitrogen R slowly through the solution. Titrate with up to 12.0 mL of 0.1 M tetrabutylammonium hydroxide and record the potentiometric titration curve (2.2.20). If the curve shows 2 points of inflexion, the volume of titrant added between the 2 points is not greater than 5.0 mL. If the curve shows no point of inflexion, the solution to be examined does not comply with the test. If the curve shows 1 point of inflexion, repeat the test but add 3.0 mL of a 25.0 g\/L solution of dimethyldecylamine R in 2-propanol R before the titration. If the titration curve after the addition of 12.0 mL of the titrant shows only 1 point of inflexion, the solution to be examined does not comply with the test.<\/p>\n Sulfated ash (2.4.14)<\/h4>\n Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n ASSAY<\/h2>\n Determine the density (2.2.5) of the solution to be examined. Dilute 4.00 g to 100.0 mL with water R. Transfer 25.0 mL of the solution to a separating funnel, add 25 mL of methylene chloride R, 10 mL of 0.1 M sodium hydroxide and 10.0 mL of a freshly prepared 50 g\/L solution of potassium iodide R. Shake well, allow to separate and discard the methylene chloride layer. Shake the aqueous layer with 3 quantities, each of 10 mL, of methylene chloride R and discard the methylene chloride layers. To the aqueous layer add 40 mL of hydrochloric acid R, allow to cool and titrate with 0.05 M potassium iodate until the deep-brown colour is almost discharged. Add 5 mL of methylene chloride R and continue the titration, shaking vigorously, until the methylene chloride layer no longer changes colour. Carry out a blank titration on a mixture of 10.0 mL of the freshly prepared 50 g\/L solution of potassium iodide R, 20 mL of water R and 40 mL of hydrochloric acid R.<\/p>\n 1 mL of 0.05 M potassium iodate is equivalent to mg of benzalkonium chloride where x is the average relative molecular mass of the sample.<\/p>\n LABELLING<\/h2>\n The label states the content of ethanol (96 per cent), if any.<\/p>\n IMPURITIES<\/h2>\n Specified impurities A, B, C.<\/p>\n $\"Benzalkonium$ <\/p>\n A. benzyl alcohol,<\/p>\n $\"Benzalkonium$ <\/p>\n B. benzaldehyde,<\/p>\n $\"Benzalkonium$ <\/p>\n C. (chloromethyl)benzene.<\/p>\n","protected":false},"excerpt":{"rendered":" (Ph. Eur. monograph 0371) Action and use Antiseptic. DEFINITION Aqueous solution of a mixture of alkylbenzyldimethylammonium chlorides, the alkyl groups mainly having chain lengths of C12, C14 and C16. Content 475 g\/L to 525 g\/L of alkylbenzyldimethylammonium chlorides, calculated using the average relative molecular mass (see Tests). The solution may contain ethanol (96 per cent)….<\/p>\n","protected":false},"author":2,"featured_media":3529,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-3491","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/3491","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=3491"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/3491\/revisions"}],"predecessor-version":[{"id":5227,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/3491\/revisions\/5227"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/3529"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=3491"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=3491"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=3491"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

Content<\/h3>\n475 g\/L to 525 g\/L of alkylbenzyldimethylammonium chlorides, calculated using the average relative molecular mass (see Tests). The solution may contain ethanol (96 per cent).<\/p>\n

CHARACTERS<\/h2>\n

Appearance<\/h3>\nClear, colourless or slightly yellowish liquid.<\/p>\n

Solubility<\/h3>\nMiscible with water and with ethanol (96 per cent).<\/p>\nIt froths copiously when shaken.<\/p>\n

TESTS<\/h2>\n

Solution S<\/h3>\nDilute 2.0 g to 100 mL with carbon dioxide-free water R.<\/p>\n

Appearance of solution<\/h3>\nSolution S is clear (2.2.1) and not more intensely coloured than reference solution Y6<\/sub> (2.2.2, Method II).<\/p>\n

Acidity or alkalinity<\/h3>\nTo 50 mL of solution S add 0.1 mL of bromocresol purple solution R. Not more than 0.1 mL of 0.1 M hydrochloric acid or 0.1 M sodium hydroxide is required to change the colour of the indicator.<\/p>\n

Sulfated ash (2.4.14)<\/h4>\nMaximum 0.1 per cent, determined on 1.0 g.<\/p>\n

LABELLING<\/h2>\nThe label states the content of ethanol (96 per cent), if any.<\/p>\n

Content<\/h3>\n
475 g\/L to 525 g\/L of alkylbenzyldimethylammonium chlorides, calculated using the average relative molecular mass (see Tests). The solution may contain ethanol (96 per cent).<\/p>\n

Appearance<\/h3>\n
Clear, colourless or slightly yellowish liquid.<\/p>\n

Solubility<\/h3>\n
Miscible with water and with ethanol (96 per cent).<\/p>\n
It froths copiously when shaken.<\/p>\n

Solution S<\/h3>\n
Dilute 2.0 g to 100 mL with carbon dioxide-free water R.<\/p>\n

Appearance of solution<\/h3>\n
Solution S is clear (2.2.1) and not more intensely coloured than reference solution Y_{6<\/sub> (2.2.2, Method II).<\/p>\n}

Acidity or alkalinity<\/h3>\n
To 50 mL of solution S add 0.1 mL of bromocresol purple solution R. Not more than 0.1 mL of 0.1 M hydrochloric acid or 0.1 M sodium hydroxide is required to change the colour of the indicator.<\/p>\n

Sulfated ash (2.4.14)<\/h4>\n
Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n

LABELLING<\/h2>\n
The label states the content of ethanol (96 per cent), if any.<\/p>\n