{"id":30889,"date":"2025-11-12T16:11:52","date_gmt":"2025-11-12T09:11:52","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=30889"},"modified":"2025-11-12T16:11:52","modified_gmt":"2025-11-12T09:11:52","slug":"thiocolchicoside-hydrate","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/thiocolchicoside-hydrate\/","title":{"rendered":"Thiocolchicoside Hydrate"},"content":{"rendered":"

Edition: BP 2025 (Ph. Eur. 11.6 update)<\/p>\n

(Ph. Eur. monograph 2814)<\/p>\n

C\u2082\u2087H\u2083\u2083NO\u2081\u2080S, xH\u2082O\u2003563.6 (anhydrous substance)\u20031622135-03-8<\/p>\n

Action and use<\/strong>
\nMuscle relaxant.<\/p>\n

Ph Eur<\/p>\n

DEFINITION<\/h2>\n

N-[(7S,12aRa)-3-(\u03b2-D-Glucopyranosyloxy)-1,2-dimethoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide.<\/p>\n

Content
\n96.0 per cent to 102.0 per cent (anhydrous substance).
\nIt contains a variable quantity of water.<\/p>\n

CHARACTERS<\/h2>\n

Appearance: Yellow crystalline powder, slightly hygroscopic.<\/p>\n

Solubility: Sparingly soluble in water, slightly soluble in ethanol (96 per cent), practically insoluble in acetone.<\/p>\n

Thiocolchicoside Hydrate<\/p>\n

mp: About 272\u202f\u00b0C.<\/p>\n

IDENTIFICATION<\/h2>\n

Infrared absorption spectrophotometry (2.2.24).<\/p>\n

Comparison thiocolchicoside hydrate CRS.<\/p>\n

TESTS<\/h2>\n

Appearance of solution<\/p>\n

The solution is clear (2.2.1).<\/p>\n

Dissolve 0.100 g in methanol R and dilute to 10 mL with the same solvent.<\/p>\n

Specific optical rotation (2.2.7)<\/p>\n

-600 to -570 (anhydrous substance).
\nDissolve 0.500 g in water R and dilute to 100.0 mL with the same solvent.<\/p>\n

Related substances<\/strong><\/p>\n

Liquid chromatography (2.2.29). Carry out the test protected from light.<\/p>\n

Test solution (a): Dissolve 20.0 mg of the substance to be examined in methanol R and dilute to 20.0 mL with the same solvent.<\/p>\n

Test solution (b): Dilute 1.0 mL of test solution (a) to 10.0 mL with methanol R.<\/p>\n

Reference solution (a): Dilute 1.0 mL of test solution (a) to 100.0 mL with methanol R. Dilute 2.0 mL of this solution to 10.0 mL with methanol R.<\/p>\n

Reference solution (b): Dissolve 5 mg of thiocolchicoside for system suitability CRS (containing impurities D, E, G, H, K and L) in methanol R and dilute to 5 mL with the same solvent.<\/p>\n

Reference solution (c): Dissolve 20.0 mg of thiocolchicoside hydrate CRS in methanol R and dilute to 20.0 mL with the same solvent. Dilute 1.0 mL of the solution to 10.0 mL with methanol R.<\/p>\n

Column:
\n\u2014 size: l = 0.10 m, \u00d8 = 2.1 mm;
\n\u2014 stationary phase: end-capped ethylene-bridged phenylsilyl silica gel for chromatography (hybrid material) R (1.7 \u00b5m);
\n\u2014 temperature: 25 \u00b0C.<\/p>\n

Mobile phase:<\/p>\n

A: dissolve 0.22 g of ammonium formate R in 350 mL of water for chromatography R and mix with 25 mL of tetrahydrofuran R;<\/p>\n

B: dilute 45 \u00b5L of anhydrous formic acid R in 900 mL of acetonitrile R and mix with 100 mL of tetrahydrofuran R;<\/p>\n\n\n\n\n\n\n\n\n\n\n
Time (min)<\/th>\nMobile phase A (per cent V\/V)<\/th>\nMobile phase B (per cent V\/V)<\/th>\n<\/tr>\n<\/thead>\n
0 – 2<\/td>\n99<\/td>\n1<\/td>\n<\/tr>\n
2 – 6<\/td>\n99 \u2192 92<\/td>\n1 \u2192 8<\/td>\n<\/tr>\n
6 – 7.5<\/td>\n92 \u2192 70<\/td>\n8 \u2192 30<\/td>\n<\/tr>\n
7.5 – 8.8<\/td>\n70 \u2192 50<\/td>\n30 \u2192 50<\/td>\n<\/tr>\n
8.8 – 9.2<\/td>\n50 \u2192 2<\/td>\n50 \u2192 98<\/td>\n<\/tr>\n
9.2 – 10<\/td>\n2<\/td>\n98<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Flow rate: 0.4 mL\/min.<\/p>\n

Detection: Spectrophotometer at 370 nm.<\/p>\n

Injection: 1.0 \u00b5L of test solution (a) and reference solutions (a) and (b).<\/p>\n

Identification of impurities: Use the chromatogram supplied with thiocolchicoside for system suitability CRS and the chromatogram obtained with reference solution (b) to identify the peaks due to impurities D, E, G, H, K and L.<\/p>\n

Relative retention:
\nWith reference to thiocolchicoside (retention time = about 6 min):
\nimpurity D = about 0.2;
\nimpurity H = about 0.4;
\nimpurity G = about 0.7;
\nimpurity K = about 0.8;
\nimpurity E = about 1.05;
\nimpurity L = about 1.11.<\/p>\n

System suitability:
\n\u2014 signal-to-noise ratio: minimum 50 for the principal peak in the chromatogram obtained with reference solution (a);
\n\u2014 peak-to-valley ratio: minimum 1.5, where Hp = height above the baseline of the peak due to impurity E and Hv = height above the baseline of the lowest point of the curve separating this peak from the peak due to thiocolchicoside in the chromatogram obtained with reference solution (b).<\/p>\n

Calculation of percentage contents:
\n\u2014 correction factors: multiply the peak areas of the following impurities by the corresponding correction factor:
\nimpurity D = 1.7;
\nimpurity H = 2.8;
\n\u2014 for each impurity, use the concentration of thiocolchicoside hydrate in reference solution (a).<\/p>\n

Limits:
\n\u2014 impurity E: maximum 1.0 per cent;
\n\u2014 impurity H: maximum 0.7 per cent;
\n\u2014 impurity K: maximum 0.3 per cent;
\n\u2014 impurities D, G: for each impurity, maximum 0.2 per cent;
\n\u2014 unspecified impurities: for each impurity, maximum 0.10 per cent;
\n\u2014 total: maximum 2.5 per cent;
\n\u2014 reporting threshold: 0.05 per cent; disregard the peak due to impurity L.<\/p>\n

Water (2.5.12)<\/p>\n

Maximum 2.5 per cent, determined on 0.500 g.<\/p>\n

Sulfated ash (2.4.14)<\/p>\n

Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n

ASSAY<\/h2>\n

Liquid chromatography (2.2.29) as described in the test for related substances with the following modification.<\/p>\n

Injection: Test solution (b) and reference solution (c).<\/p>\n

Calculate the percentage content of C\u2082\u2087H\u2083\u2083NO\u2081\u2080S taking into account the assigned content of thiocolchicoside hydrate CRS.<\/p>\n

STORAGE<\/h2>\n

In an airtight container, protected from light.<\/p>\n

IMPURITIES<\/h2>\n

Specified impurities: D, E, G, H, K.<\/p>\n

Other detectable impurities (the following substances would, if present at a sufficient level, be detected by one or other of the tests in the monograph. They are limited by the general acceptance criterion for other\/unspecified impurities and\/or by the general monograph Substances for pharmaceutical use (2034). It is therefore not necessary to identify these impurities for demonstration of compliance. See also 5.10. Control of impurities in substances for pharmaceutical use):<\/p>\n

\"\"
A. N-[(7S,12aRa)-1,2,3,10-tetramethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (colchicine)<\/figcaption><\/figure>\n
\"\"
B. N-[(7S,12aRa)-1,2,3-trimethoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (thiocolchicine)<\/figcaption><\/figure>\n
\"\"
C. N-[(7S,12aRa)-3-hydroxy-1,2-dimethoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (3-O-demethylthiocolchicine)<\/figcaption><\/figure>\n
\"\"
D. N-[(7S,12aRa)-3-(\u03b2-D-glucopyranosyloxy)-1,2,10-trimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (colchicoside)<\/figcaption><\/figure>\n
\"\"
E. N-[(7S,12aRa)-3-(\u03b2-D-glucopyranosyloxy)-1,2-dimethoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]formamide (N-deacetyl-N-formylthiocolchicoside)<\/figcaption><\/figure>
\"\"
G. (7S,12aRa)-7-amino-3-(\u03b2-D-glucopyranosyloxy)-1,2-dimethoxy-10-(methylsulfanyl)-6,7-dihydrobenzo[a]heptalen-9(5H)-one (N-deacetylthiocolchicoside)<\/figcaption><\/figure>
\"\"
H. N-[(7S,12aRa)-3-(\u03b2-D-glucopyranosyloxy)-1,2-dimethoxy-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (10-demethoxycolchicoside)<\/figcaption><\/figure> <\/p>\n
\"\"
J. N-[(7S,12aRa)-3-(\u03b2-D-glucopyranosyloxy)-1,2-dimethoxy-10-(methylsulfinyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (thiocolchicoside S-oxide)<\/figcaption><\/figure>
\"\"
K. N-[(7S,12aRa)-3-(\u03b2-D-glucopyranosyloxy)-2-hydroxy-1-methoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide or N-[(7S,12aRa)-3-(\u03b2-D-glucopyranosyloxy)-1-hydroxy-2-methoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide<\/figcaption><\/figure> <\/p>\n
\"\"
L. N-[(7S,12aSa)-3-(\u03b2-D-glucopyranosyloxy)-1,2-dimethoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide (12aSa-thiocolchicoside)<\/figcaption><\/figure>\n","protected":false},"excerpt":{"rendered":"

Edition: BP 2025 (Ph. Eur. 11.6 update) (Ph. Eur. monograph 2814) C\u2082\u2087H\u2083\u2083NO\u2081\u2080S, xH\u2082O\u2003563.6 (anhydrous substance)\u20031622135-03-8 Action and use Muscle relaxant. Ph Eur DEFINITION N-[(7S,12aRa)-3-(\u03b2-D-Glucopyranosyloxy)-1,2-dimethoxy-10-(methylsulfanyl)-9-oxo-5,6,7,9-tetrahydrobenzo[a]heptalen-7-yl]acetamide. Content 96.0 per cent to 102.0 per cent (anhydrous substance). It contains a variable quantity of water. CHARACTERS Appearance: Yellow crystalline powder, slightly hygroscopic. Solubility: Sparingly soluble in water, slightly soluble…<\/p>\n","protected":false},"author":5,"featured_media":30890,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-30889","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/30889","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/5"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=30889"}],"version-history":[{"count":1,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/30889\/revisions"}],"predecessor-version":[{"id":30911,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/30889\/revisions\/30911"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/30890"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=30889"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=30889"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=30889"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}