{"id":2684,"date":"2025-09-22T14:33:32","date_gmt":"2025-09-22T07:33:32","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=2684"},"modified":"2025-10-02T11:47:15","modified_gmt":"2025-10-02T04:47:15","slug":"benzethonium-chloride","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/benzethonium-chloride\/","title":{"rendered":"Benzethonium Chloride"},"content":{"rendered":"

(Ph. Eur. monograph 0974)<\/p>\n

C_{27<\/sub>H_{42<\/sub>ClNO_{2<\/sub>\u00a0 \u00a0 448.1\u00a0 \u00a0 121-54-0<\/p>\n}}}

Action and use<\/strong><\/p>\n

Antiseptic.<\/p>\n

DEFINITION<\/h2>\n
N-Benzyl-N,N-dimethyl-2-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethanaminium chloride.<\/p>\n
Content<\/h3>\n
97.0 per cent to 103.0 per cent (dried substance).<\/p>\n
CHARACTERS<\/h2>\n
Appearance<\/h3>\n
White or yellowish-white powder.<\/p>\n
Solubility<\/h3>\n
Very soluble in water and in ethanol (96 per cent), freely soluble in methylene chloride.<\/p>\n
An aqueous solution froths copiously when shaken.<\/p>\n
IDENTIFICATION<\/h2>\n
A. Melting point (2.2.14): 158 \u00b0C to 164 \u00b0C, after drying at 105 \u00b0C for 4 h.<\/p>\n
B. Thin-layer chromatography (2.2.27).<\/p>\n
Test solution: Dissolve 25 mg of the substance to be examined in water R and dilute to 5 mL with the same solvent.<\/p>\n
Reference solution: Dissolve 25 mg of benzethonium chloride CRS in water R and dilute to 5 mL with the same solvent.<\/p>\n
Plate: TLC silica gel F254 plate R.<\/p>\n
Mobile phase glacial acetic acid R, water R, methanol R (5:5:100 V\/V\/V).<\/p>\n
Application: 20 \u03bcL.<\/p>\n
Development: Over a path of 12 cm.<\/p>\n
Drying In: a current of warm air.<\/p>\n
Detection: Examine in ultraviolet light at 254 nm.<\/p>\n
Results: The principal spot in the chromatogram obtained with the test solution is similar in position and size to the principal spot in the chromatogram obtained with the reference solution.<\/p>\n
C. To 5 mL of dilute sodium hydroxide solution R add 0.1 mL of bromophenol blue solution R1 and 5 mL of methylene chloride R and shake. The lower layer is colourless. Add 0.1 mL of solution S (see Tests) and shake. A blue colour develops in the lower layer.<\/p>\n
D. To 2 mL of solution S add 1 mL of dilute nitric acid R. A white precipitate is formed which dissolves upon addition of 5 mL of ethanol (96 per cent) R. The solution gives reaction (a) of chlorides (2.3.1).<\/p>\n
TESTS<\/h2>\n
Solution S<\/h3>\n
Dissolve 5.0 g in carbon dioxide-free water R and dilute to 50 mL with the same solvent.<\/p>\n
Appearance of solution<\/h3>\n
Solution S is clear (2.2.1) and not more intensely coloured than reference solution Y6 (2.2.2, Method II).<\/p>\n
Acidity or alkalinity<\/h3>\n
To 25 mL of solution S add 0.1 mL of phenolphthalein solution R. The solution is colourless. Add 0.3 mL of 0.01 M sodium hydroxide. The solution is pink. Add 0.1 mL of methyl red solution R and 0.5 mL of 0.01 M hydrochloric acid. The solution is orange-red.<\/p>\n
Volatile bases and salts of volatile bases (2.4.1, Method B)<\/h3>\n
Maximum 50 ppm, determined on 0.20 g.<\/p>\n
Prepare the standard using 0.1 mL of ammonium standard solution (100 ppm NH4) R. Replace heavy magnesium oxide by 2.0 mL of strong sodium hydroxide solution R.<\/p>\n
Loss on drying (2.2.32)<\/h4>\n
Maximum 5.0 per cent, determined on 1.000 g by drying in an oven at 105 \u00b0C for 4 h.<\/p>\n
Sulfated ash (2.4.14)<\/h4>\n
Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n
ASSAY<\/h2>\n
Dissolve 2.000 g in water R and dilute to 100.0 mL with the same solvent. Transfer 25.0 mL of the solution to a separating funnel, add 10 mL of a 4 g\/L solution of sodium hydroxide R, 10.0 mL of a freshly prepared 50 g\/L solution of potassium iodide R and 25 mL of methylene chloride R. Shake vigorously, allow to separate and discard the lower layer. Shake the upper layer with 3 quantities, each of 10 mL, of methylene chloride R and discard the lower layers. To the upper layer add 40 mL of hydrochloric acid R, allow to cool and titrate with 0.05 M potassium iodate until the deep brown colour is almost discharged. Add 4 mL of methylene chloride R and continue the titration, shaking vigorously, until the lower layer is no longer brown. Carry out a blank titration using a mixture of 10.0 mL of a freshly prepared 50 g\/L solution of potassium iodide R, 20 mL of water R and 40 mL of hydrochloric acid R.<\/p>\n
1 mL of 0.05 M potassium iodate is equivalent to 44.81 mg of C_{27<\/sub>H_{42<\/sub>ClNO_{2<\/sub>.<\/p>\n}}}
STORAGE<\/p>\n
Protected from light.<\/p>\n","protected":false},"excerpt":{"rendered":"
(Ph. Eur. monograph 0974) C27H42ClNO2\u00a0 \u00a0 448.1\u00a0 \u00a0 121-54-0 Action and use Antiseptic. DEFINITION N-Benzyl-N,N-dimethyl-2-[2-[4-(1,1,3,3-tetramethylbutyl)phenoxy]ethoxy]ethanaminium chloride. Content 97.0 per cent to 103.0 per cent (dried substance). CHARACTERS Appearance White or yellowish-white powder. Solubility Very soluble in water and in ethanol (96 per cent), freely soluble in methylene chloride. An aqueous solution froths copiously when shaken….<\/p>\n","protected":false},"author":3,"featured_media":2691,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-2684","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/2684","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=2684"}],"version-history":[{"count":3,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/2684\/revisions"}],"predecessor-version":[{"id":5516,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/2684\/revisions\/5516"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/2691"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=2684"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=2684"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=2684"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

Content<\/h3>\n97.0 per cent to 103.0 per cent (dried substance).<\/p>\n

CHARACTERS<\/h2>\n

Appearance<\/h3>\nWhite or yellowish-white powder.<\/p>\n

Solubility<\/h3>\nVery soluble in water and in ethanol (96 per cent), freely soluble in methylene chloride.<\/p>\nAn aqueous solution froths copiously when shaken.<\/p>\n

TESTS<\/h2>\n

Solution S<\/h3>\nDissolve 5.0 g in carbon dioxide-free water R and dilute to 50 mL with the same solvent.<\/p>\n

Appearance of solution<\/h3>\nSolution S is clear (2.2.1) and not more intensely coloured than reference solution Y6 (2.2.2, Method II).<\/p>\n

Acidity or alkalinity<\/h3>\nTo 25 mL of solution S add 0.1 mL of phenolphthalein solution R. The solution is colourless. Add 0.3 mL of 0.01 M sodium hydroxide. The solution is pink. Add 0.1 mL of methyl red solution R and 0.5 mL of 0.01 M hydrochloric acid. The solution is orange-red.<\/p>\n

Volatile bases and salts of volatile bases (2.4.1, Method B)<\/h3>\nMaximum 50 ppm, determined on 0.20 g.<\/p>\nPrepare the standard using 0.1 mL of ammonium standard solution (100 ppm NH4) R. Replace heavy magnesium oxide by 2.0 mL of strong sodium hydroxide solution R.<\/p>\n

Loss on drying (2.2.32)<\/h4>\nMaximum 5.0 per cent, determined on 1.000 g by drying in an oven at 105 \u00b0C for 4 h.<\/p>\n

Sulfated ash (2.4.14)<\/h4>\nMaximum 0.1 per cent, determined on 1.0 g.<\/p>\n

Content<\/h3>\n
97.0 per cent to 103.0 per cent (dried substance).<\/p>\n

Appearance<\/h3>\n
White or yellowish-white powder.<\/p>\n

Solubility<\/h3>\n
Very soluble in water and in ethanol (96 per cent), freely soluble in methylene chloride.<\/p>\n
An aqueous solution froths copiously when shaken.<\/p>\n

Solution S<\/h3>\n
Dissolve 5.0 g in carbon dioxide-free water R and dilute to 50 mL with the same solvent.<\/p>\n

Appearance of solution<\/h3>\n
Solution S is clear (2.2.1) and not more intensely coloured than reference solution Y6 (2.2.2, Method II).<\/p>\n

Acidity or alkalinity<\/h3>\n
To 25 mL of solution S add 0.1 mL of phenolphthalein solution R. The solution is colourless. Add 0.3 mL of 0.01 M sodium hydroxide. The solution is pink. Add 0.1 mL of methyl red solution R and 0.5 mL of 0.01 M hydrochloric acid. The solution is orange-red.<\/p>\n

Volatile bases and salts of volatile bases (2.4.1, Method B)<\/h3>\n
Maximum 50 ppm, determined on 0.20 g.<\/p>\n
Prepare the standard using 0.1 mL of ammonium standard solution (100 ppm NH4) R. Replace heavy magnesium oxide by 2.0 mL of strong sodium hydroxide solution R.<\/p>\n

Loss on drying (2.2.32)<\/h4>\n
Maximum 5.0 per cent, determined on 1.000 g by drying in an oven at 105 \u00b0C for 4 h.<\/p>\n

Sulfated ash (2.4.14)<\/h4>\n
Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n