{"id":23118,"date":"2025-10-31T11:30:03","date_gmt":"2025-10-31T04:30:03","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=23118"},"modified":"2025-10-31T11:30:03","modified_gmt":"2025-10-31T04:30:03","slug":"enilconazole","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/enilconazole\/","title":{"rendered":"Enilconazole"},"content":{"rendered":"

(Enilconazole for Veterinary Use, Ph. Eur. monograph 1720)<\/p>\n

C14H14Cl2N2O 297.2 35554-44-0<\/p>\n

Action and use<\/p>\n

Antifungal.<\/p>\n

DEFINITION<\/p>\n

1-[(2RS)-2-(2,4-Dichlorophenyl)-2-(prop-2-enyloxy)ethyl]-1H-imidazole.<\/p>\n

Content<\/p>\n

98.5 per cent to 101.5 per cent (dried substance).<\/p>\n

CHARACTERS<\/p>\n

Appearance<\/p>\n

Clear, yellowish, oily liquid or solid mass.<\/p>\n

Solubility<\/p>\n

Very slightly soluble in water, freely soluble in ethanol (96 per cent), in methanol and in toluene.<\/p>\n

IDENTIFICATION<\/p>\n

Infrared absorption spectrophotometry (2.2.24).<\/p>\n

Comparison enilconazole CRS.<\/p>\n

TESTS<\/p>\n

Optical rotation (2.2.7)<\/p>\n

-0.10\u00b0 to + 0.10\u00b0.<\/p>\n

Dissolve 0.1 g in methanol R and dilute to 10 mL with the same solvent.<\/p>\n

Related substances<\/p>\n

Gas chromatography (2.2.28). Prepare the solutions immediately before use and protect from light.<\/p>\n

Test solution Dissolve 0.100 g of the substance to be examined in toluene R and dilute to 100.0 mL with the same solvent.<\/p>\n

Reference solution (a) Dissolve 10 mg of enilconazole CRS and 10 mg of enilconazole impurity E CRS in toluene R and dilute to 100 mL with the same solvent.<\/p>\n

Reference solution (b) Dilute 5.0 mL of the test solution to 100.0 mL with toluene R. Dilute 1.0 mL of this solution to 10.0 mL with toluene R.<\/p>\n

Column:<\/p>\n

\u2014 material: fused silica;<\/p>\n

\u2014 size: l = 25 m, \u00d8 = 0.32 mm;<\/p>\n

\u2014 stationary phase: phenyl(5)methyl(95)polysiloxane R (film thickness 0.52 \u03bcm).<\/p>\n

Carrier gas helium for chromatography R.<\/p>\n

Flow rate 1.3 mL\/min.<\/p>\n

Split ratio 1:38.<\/p>\n

Temperature:<\/p>\n\n\n\n\n\n\n\n
<\/td>\n Time
\n(min)<\/td>\n Temperature
\n(\u00b0C)<\/td>\n<\/tr>\n
Column<\/td>\n 0 – 6.4<\/td>\n 100 \u2192 260<\/td>\n<\/tr>\n
<\/td>\n 6.4 – 14<\/td>\n 260<\/td>\n<\/tr>\n
Injection port<\/td>\n <\/td>\n 250<\/td>\n<\/tr>\n
Detector<\/td>\n <\/td>\n 300<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n
Detection Flame ionisation.<\/p>\n
Injection 2 \u03bcL.<\/p>\n
Identification of impurities Use the chromatogram obtained with reference solution (a) to identify the peak due to impurity E.<\/p>\n
Relative retention With reference to enilconazole (retention time = about 10 min): impurity A = about 0.6; impurity B = about 0.7; impurity C = about 0.8; impurity D = about 0.9; impurity E = about 1.03; impurity F = about 1.1.<\/p>\n
System suitability Reference solution (a):<\/p>\n
\u2014 resolution: minimum 2.5 between the peaks due to enilconazole and impurity E.<\/p>\n
Limits:<\/p>\n
\u2014 impurities A, B, C, D, E, F: for each impurity, not more than twice the area of the principal peak in the chromatogram obtained with reference solution (b) (1.0 per cent), and not more than 1 such peak has an area greaterthan the area of the principal peak in the chromatogram obtained with reference solution (b) (0.5 per cent);<\/p>\n
\u2014 unspecified impurities: for each impurity, not more than 0.4 times the area of the principal peak in the chromatogram obtained with reference solution (b) (0.20 per cent);<\/p>\n
\u2014 total: not more than 4 times the area of the principal peak in the chromatogram obtained with reference solution (b) (2.0 per cent);<\/p>\n
\u2014 disregard limit: 0.1 times the area of the principal peak in the chromatogram obtained with reference solution (b) (0.05 per cent).<\/p>\n
Loss on drying (2.2.32)<\/p>\n
Maximum 0.5 per cent, determined on 1.000 g by drying in vacuo at 40 \u00b0C for 4 h.<\/p>\n
Sulfated ash (2.4.14)<\/p>\n
Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n
ASSAY<\/p>\n
Dissolve 0.230 g in 50 mL of a mixture of 1 volume of anhydrous acetic acid R and 7 volumes of methyl ethyl ketone R. Titrate with 0.1 M perchloric acid using 0.2 mL of naphtholbenzein solution R as indicator.<\/p>\n
1 mL of 0.1 M perchloric acid is equivalent to 29.72 mg of C14H14Cl2N2O.<\/p>\n
STORAGE<\/p>\n
In an airtight container, protected from light.<\/p>\n
IMPURITIES<\/p>\n
Specified impurities A, B, C, D, E, F.<\/p>\n
$\"Enilconazole\"$ <\/p>\n
A. (2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethanamine,<\/p>\n
$\"Enilconazole\"$ <\/p>\n
B. N-[(2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]prop-2-en-1-amine,<\/p>\n
$\"Enilconazole\"$ <\/p>\n
C. N-[(2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]formamide,<\/p>\n
$\"Enilconazole\"$ <\/p>\n
D. N-[(2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]-N-(prop-2-enyl)formamide,<\/p>\n
$\"Enilconazole\"$ <\/p>\n
E. (1RS)-1-(2,4-dichlorophenyl)-2-(-1H-imidazol-1-yl)ethanol,<\/p>\n
$\"Enilconazole\"$ <\/p>\n
F. 1-[(2RS)-2-(3,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]-1H-imidazole.<\/p>\n","protected":false},"excerpt":{"rendered":"
(Enilconazole for Veterinary Use, Ph. Eur. monograph 1720) C14H14Cl2N2O 297.2 35554-44-0 Action and use Antifungal. DEFINITION 1-[(2RS)-2-(2,4-Dichlorophenyl)-2-(prop-2-enyloxy)ethyl]-1H-imidazole. Content 98.5 per cent to 101.5 per cent (dried substance). CHARACTERS Appearance Clear, yellowish, oily liquid or solid mass. Solubility Very slightly soluble in water, freely soluble in ethanol (96 per cent), in methanol and in toluene. IDENTIFICATION…<\/p>\n","protected":false},"author":2,"featured_media":23125,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[176],"tags":[],"class_list":["post-23118","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-british-pharmacopoeia-veterinary-2020"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/23118","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=23118"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/23118\/revisions"}],"predecessor-version":[{"id":23128,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/23118\/revisions\/23128"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/23125"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=23118"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=23118"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=23118"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}

<\/td>\n	Time \n(min)<\/td>\n	Temperature \n(\u00b0C)<\/td>\n<\/tr>\n
Column<\/td>\n	0 – 6.4<\/td>\n	100 \u2192 260<\/td>\n<\/tr>\n
<\/td>\n	6.4 – 14<\/td>\n	260<\/td>\n<\/tr>\n
Injection port<\/td>\n	<\/td>\n	250<\/td>\n<\/tr>\n
Detector<\/td>\n	<\/td>\n	300<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n Detection Flame ionisation.<\/p>\n Injection 2 \u03bcL.<\/p>\n Identification of impurities Use the chromatogram obtained with reference solution (a) to identify the peak due to impurity E.<\/p>\n Relative retention With reference to enilconazole (retention time = about 10 min): impurity A = about 0.6; impurity B = about 0.7; impurity C = about 0.8; impurity D = about 0.9; impurity E = about 1.03; impurity F = about 1.1.<\/p>\n System suitability Reference solution (a):<\/p>\n \u2014 resolution: minimum 2.5 between the peaks due to enilconazole and impurity E.<\/p>\n Limits:<\/p>\n \u2014 impurities A, B, C, D, E, F: for each impurity, not more than twice the area of the principal peak in the chromatogram obtained with reference solution (b) (1.0 per cent), and not more than 1 such peak has an area greaterthan the area of the principal peak in the chromatogram obtained with reference solution (b) (0.5 per cent);<\/p>\n \u2014 unspecified impurities: for each impurity, not more than 0.4 times the area of the principal peak in the chromatogram obtained with reference solution (b) (0.20 per cent);<\/p>\n \u2014 total: not more than 4 times the area of the principal peak in the chromatogram obtained with reference solution (b) (2.0 per cent);<\/p>\n \u2014 disregard limit: 0.1 times the area of the principal peak in the chromatogram obtained with reference solution (b) (0.05 per cent).<\/p>\n Loss on drying (2.2.32)<\/p>\n Maximum 0.5 per cent, determined on 1.000 g by drying in vacuo at 40 \u00b0C for 4 h.<\/p>\n Sulfated ash (2.4.14)<\/p>\n Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n ASSAY<\/p>\n Dissolve 0.230 g in 50 mL of a mixture of 1 volume of anhydrous acetic acid R and 7 volumes of methyl ethyl ketone R. Titrate with 0.1 M perchloric acid using 0.2 mL of naphtholbenzein solution R as indicator.<\/p>\n 1 mL of 0.1 M perchloric acid is equivalent to 29.72 mg of C14H14Cl2N2O.<\/p>\n STORAGE<\/p>\n In an airtight container, protected from light.<\/p>\n IMPURITIES<\/p>\n Specified impurities A, B, C, D, E, F.<\/p>\n $\"Enilconazole\"$ <\/p>\n A. (2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethanamine,<\/p>\n $\"Enilconazole\"$ <\/p>\n B. N-[(2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]prop-2-en-1-amine,<\/p>\n $\"Enilconazole\"$ <\/p>\n C. N-[(2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]formamide,<\/p>\n $\"Enilconazole\"$ <\/p>\n D. N-[(2RS)-2-(2,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]-N-(prop-2-enyl)formamide,<\/p>\n $\"Enilconazole\"$ <\/p>\n E. (1RS)-1-(2,4-dichlorophenyl)-2-(-1H-imidazol-1-yl)ethanol,<\/p>\n $\"Enilconazole\"$ <\/p>\n F. 1-[(2RS)-2-(3,4-dichlorophenyl)-2-(prop-2-enyloxy)ethyl]-1H-imidazole.<\/p>\n","protected":false},"excerpt":{"rendered":" (Enilconazole for Veterinary Use, Ph. Eur. monograph 1720) C14H14Cl2N2O 297.2 35554-44-0 Action and use Antifungal. DEFINITION 1-[(2RS)-2-(2,4-Dichlorophenyl)-2-(prop-2-enyloxy)ethyl]-1H-imidazole. Content 98.5 per cent to 101.5 per cent (dried substance). CHARACTERS Appearance Clear, yellowish, oily liquid or solid mass. Solubility Very slightly soluble in water, freely soluble in ethanol (96 per cent), in methanol and in toluene. IDENTIFICATION…<\/p>\n","protected":false},"author":2,"featured_media":23125,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[176],"tags":[],"class_list":["post-23118","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-british-pharmacopoeia-veterinary-2020"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/23118","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=23118"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/23118\/revisions"}],"predecessor-version":[{"id":23128,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/23118\/revisions\/23128"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/23125"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=23118"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=23118"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=23118"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}