{"id":22422,"date":"2025-10-30T09:56:15","date_gmt":"2025-10-30T02:56:15","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=22422"},"modified":"2025-10-30T09:56:15","modified_gmt":"2025-10-30T02:56:15","slug":"amitraz","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/amitraz\/","title":{"rendered":"Amitraz"},"content":{"rendered":"

C19<\/sub>H23<\/sub>N3\u00a0<\/sub> \u00a0 \u00a0 \u00a0293.4\u00a0 \u00a0 \u00a0 \u00a033089-61-1<\/p>\n

Action and use<\/strong><\/p>\n

Topical parasiticide; acaricide.<\/p>\n

Preparation<\/strong><\/p>\n

Amitraz Dip Concentrate (Liquid)<\/p>\n

DEFINITION<\/h2>\n

Amitraz is N-methylbis (2,4-xylyliminomethyl) amine. It contains not less than 97.0% and not more than 101.0% of C19<\/sub>H23<\/sub>N3<\/sub>, calculated with reference to the anhydrous substance.<\/p>\n

CHARACTERISTICS<\/h2>\n

A white to buff powder.<\/p>\n

Practically insoluble in water; decomposes slowly in ethanol (96%); freely soluble in acetone.<\/p>\n

IDENTIFICATION<\/h2>\n

The infrared absorption spectrum, Appendix II A, is concordant with the reference spectrum of amitraz (RSV 04).<\/p>\n

TESTS<\/h2>\n

Related substances<\/h3>\n

Carry out the method for gas chromatography, Appendix III B, using the following solutions.<\/p>\n

0.010% w\/v of 2,4-dimethylaniline, 0.20% w\/v of form-2\u2032,4\u2032-xylidide BPCRS and 0.20% w\/v of N,N\u2032-bis(2,4-xylyl)formamidine BPCRS in methyl acetate (solution A)<\/p>\n

Disperse 30 mg of N-methyl-N\u2032-(2,4-xylyl)formamidine hydrochloride BPCRS in 5 mL of methyl acetate, add about 32 mg of triethylamine, mix with the aid of ultrasound for 2 minutes, filter, wash the filter with a small amount of methyl acetate and add sufficient methyl acetate to the combined filtrate and washings to produce 25 mL (solution B) (about 0.1% w\/v of N-methyl-N\u2032- (2,4-xylyl)formamidine).<\/p>\n

(1) 5.0% w\/v solution of the substance being examined in methyl acetate.<\/p>\n

(2) A mixture of equal volumes of solution A and solution B.<\/p>\n

CHROMATOGRAPHIC CONDITIONS<\/p>\n

(a) Use a fused silica capillary column (10 m \u00d7 0.53 mm) bonded with a film (5 \u03bcm) of poly [methyl(95)phenyl(5)]siloxane (Chrompack CP-SIL 8 CB is suitable).<\/p>\n

(b) Use helium as the carrier gas at 12 mL per minute.<\/p>\n

(c) Use gradient conditions at an initial temperature of 125\u00b0, maintained at 125\u00b0 for 5 minutes, increasing linearly to 270\u00b0 at a rate of 5\u00b0 per minute and maintained at 270\u00b0 for 15 minutes.<\/p>\n

(d) Use an inlet temperature of 230\u00b0.<\/p>\n

(e) Use a flame ionisation detector at a temperature of 300\u00b0.<\/p>\n

(f) Inject 1 \u03bcL of each of solutions (1) and (2).<\/p>\n

In the chromatogram obtained with solution (2) the peaks following the solvent peak, in order of emergence, are due to 2,4-dimethylaniline, form-2\u2032,4\u2032-xylidide, N-methyl-N\u2032-(2,4- xylyl)formamidine and N,N\u2032-bis(2,4-xylyl)formamidine.<\/p>\n

LIMITS<\/p>\n

In the chromatogram obtained with solution (1):<\/p>\n

the area of any peak corresponding to 2,4-dimethylaniline, form-2\u2032,4\u2032-xylidide, N-methyl-N\u2032-(2,4- xylyl)formamidine and N,N\u2032-bis(2,4-xylyl)formamidine is not greater than the area of the corresponding peak in the chromatogram obtained with solution (2) (0.1%, 2%, 1% and 2% respectively);<\/p>\n

the area of any other secondary peak is not greater than the area of the peak due to 2,4- dimethylaniline in the chromatogram obtained with solution (2) (0.1%).<\/p>\n

Water<\/h4>\n

Not more than 0.1% w\/w, Appendix IX C, Method IA. Use 5 g and a mixture of equal volumes of chloroform and 2-chloroethanol in place of anhydrous methanol.<\/p>\n

Sulfated ash<\/h4>\n

Not more than 0.2%, Appendix IX A.<\/p>\n

ASSAY<\/h2>\n

Carry out the method for gas chromatography, Appendix III B, using the following solutions.<\/p>\n

Prepare a 2% v\/v solution of squalane (internal standard) in methyl acetate (solution C).<\/p>\n

(1) 0.15 g of the substance being examined in sufficient methyl acetate to produce 30 mL.<\/p>\n

(2) 0.15 g of the substance being examined in 10 mL of solution C and add sufficient methyl acetate to produce 30 mL.<\/p>\n

(3) 1.50% w\/v solution of amitraz BPCRS in solution C and dilute 1 volume of this solution to 3 volumes with methyl acetate.<\/p>\n

CHROMATOGRAPHIC CONDITIONS<\/p>\n

(a) Use a fused silica capillary column (15 m \u00d7 0.53 mm) coated with a 1.5 \u03bcm film of methyl silicone gum (Chrompack CP-Sil 5 CB is suitable).<\/p>\n

(b) Use helium as the carrier gas at 12 mL per minute.<\/p>\n

(c) Use isothermal conditions maintained at 220\u00b0.<\/p>\n

(d) Use an inlet temperature of 230\u00b0.<\/p>\n

(e) Use a flame ionisation detector at a temperature of 300\u00b0.<\/p>\n

(f) Inject 1 \u03bcL of each solution.<\/p>\n

SYSTEM SUITABILITY<\/p>\n

The assay is not valid unless, in the chromatogram obtained with solution (3), the resolution factor between the peaks corresponding to squalane and amitraz is at least 3.0.<\/p>\n

LIMITS<\/p>\n

Calculate the content o fC19<\/sub>H23<\/sub>N3 <\/sub>from the chromatograms obtained using the declared content of C19<\/sub>H23<\/sub>N3 <\/sub>in amitraz BPCRS.<\/p>\n

STORAGE<\/h2>\n

Amitraz should be kept in a well-closed container, which may contain paraformaldehyde, packed in separate sachets as a stabiliser.<\/p>\n

\"Amitraz\"<\/p>\n

A. 2,4-dimethylaniline (2,4-xylidine),<\/p>\n

\"Amitraz\"<\/p>\n

B. form-2\u2032,4\u2032-xylidide,<\/p>\n

\"Amitraz\"<\/p>\n

C. N-methyl-N\u2032-(2,4-xylyl)formamidine,<\/p>\n

\"Amitraz\"<\/p>\n

D. N,N\u2032-bis(2,4-xylyl)formamidine.<\/p>\n","protected":false},"excerpt":{"rendered":"

C19H23N3\u00a0 \u00a0 \u00a0 \u00a0293.4\u00a0 \u00a0 \u00a0 \u00a033089-61-1 Action and use Topical parasiticide; acaricide. Preparation Amitraz Dip Concentrate (Liquid) DEFINITION Amitraz is N-methylbis (2,4-xylyliminomethyl) amine. It contains not less than 97.0% and not more than 101.0% of C19H23N3, calculated with reference to the anhydrous substance. CHARACTERISTICS A white to buff powder. Practically insoluble in water; decomposes…<\/p>\n","protected":false},"author":2,"featured_media":22434,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[176],"tags":[],"class_list":["post-22422","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-british-pharmacopoeia-veterinary-2020"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/22422","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=22422"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/22422\/revisions"}],"predecessor-version":[{"id":22436,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/22422\/revisions\/22436"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/22434"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=22422"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=22422"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=22422"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}