(Ph. Eur. monograph 1647)<\/p>\n
C12<\/sub>H22<\/sub>O12<\/sub> (acid form)\u00a0 \u00a0 \u00a0 \u00a0358.3\u00a0 \u00a0 \u00a0 \u00a096-82-2<\/p>\n C12<\/sub>H20<\/sub>O11<\/sub>\u00a0(\u03b4-lactone)\u00a0 \u00a0 \u00a0 \u00a0340.3\u00a0 \u00a0 \u00a0 \u00a05965-65-1<\/p>\n Mixture in variable proportions of 4-O-\u03b2-D-galactopyranosyl-D-gluconic acid and 4-O-\u03b2-D-galactopyranosyl-D-glucono-1,5-lactone.<\/p>\n 98.0 per cent to 102.0 per cent (anhydrous substance).<\/p>\n White or almost white powder.<\/p>\n Freely soluble in water, slightly soluble in glacial acetic acid, in anhydrous ethanol and in methanol.<\/p>\n About 125 \u00b0C with decomposition.<\/p>\n A. Infrared absorption spectrophotometry (2.2.24).<\/p>\n Comparison: lactobionic acid CRS.<\/p>\n If the spectra obtained show differences, dissolve the substance to be examined and the reference substance separately in water R, dry at 105 \u00b0C and record new spectra using the residues.<\/p>\n B. Thin-layer chromatography (2.2.27).<\/p>\n Test solution: Dissolve 10 mg of the substance to be examined in water R and dilute to 1 mL with the same solvent.<\/p>\n Reference solution: Dissolve 10 mg of lactobionic acid CRS in water R and dilute to 1 mL with the same solvent.<\/p>\n Plate: TLC silica gel plate R.<\/p>\n Mobile phase: concentrated ammonia R1, ethyl acetate R, water R, methanol R (2:2:2:4 V\/V\/V\/V).<\/p>\n Application:5 \u03bcL.<\/p>\n Development: Over 3\/4 of the plate.<\/p>\n Detection: Spray 3 times with ammonium molybdate solution R6 and heat in an oven at 110 \u00b0C for 15 min.<\/p>\n Results: The principal spot in the chromatogram obtained with the test solution is similar in position and colour to the principal spot in the chromatogram obtained with the reference solution.<\/p>\n The solution is clear (2.2.1) and not more intensely coloured than reference solution Y5<\/sub> (2.2.2, Method II).<\/p>\n Dissolve 3.0 g in 25 mL of water R.<\/p>\n + 23.0 to + 29.0 (anhydrous substance).<\/p>\n Dissolve 1.0 g in 80 mL of water R and dilute to 100.0 mL with the same solvent.<\/p>\n Allow to stand for 24 h.<\/p>\n Maximum 0.2 per cent, calculated as glucose.<\/p>\n Dissolve 5.0 g in 25 mL of water R with the aid of gentle heat. Cool and add 20 mL of cupri-citric solution R and a few glass beads. Heat so that boiling begins after 4 min and maintain boiling for 3 min. Cool rapidly and add 100 mL of a 2.4 per cent V\/V solution of glacial acetic acid R and 20.0 mL of 0.025 M iodine. With continuous shaking, add 25 mL of a mixture of 6 volumes of hydrochloric acid R and 94 volumes of water R and, when the precipitate has dissolved, titrate the excess of iodine with 0.05 M sodium thiosulfate using 1 mL of starch solution R, added towards the end of the titration, as indicator. Not less than 12.8 mL of 0.05 M sodium thiosulfate is required.<\/p>\n Maximum 5.0 per cent, determined on 0.50 g.<\/p>\n Use a mixture of 1 volume of formamide R and 2 volumes of methanol R as solvent.<\/p>\n Maximum 0.2 per cent.<\/p>\n Dissolve 0.350 g in 50 mL of carbon dioxide-free water R, previously heated to 30 \u00b0C. Immediately titrate with 0.1 M sodium hydroxide and determine the 2 equivalence points potentiometrically (2.2.20).<\/p>\n The first equivalence point (V1<\/sub>) corresponds to the acid form of lactobionic acid and the second equivalence point (V2<\/sub> -V1<\/sub>) corresponds to the \u03b4-lactone form.<\/p>\n 1 mL of 0.1 M sodium hydroxide is equivalent to 35.83 mg of C12<\/sub>H22<\/sub>O12<\/sub>.<\/p>\n 1 mL of 0.1 M sodium hydroxide is equivalent to 34.03 mg of C12<\/sub>H20<\/sub>O11<\/sub>.<\/p>\n The sum of the 2 results is expressed as a percentage content of lactobionic acid.<\/p>\n","protected":false},"excerpt":{"rendered":" (Ph. Eur. monograph 1647) C12H22O12 (acid form)\u00a0 \u00a0 \u00a0 \u00a0358.3\u00a0 \u00a0 \u00a0 \u00a096-82-2 C12H20O11\u00a0(\u03b4-lactone)\u00a0 \u00a0 \u00a0 \u00a0340.3\u00a0 \u00a0 \u00a0 \u00a05965-65-1 DEFINITION Mixture in variable proportions of 4-O-\u03b2-D-galactopyranosyl-D-gluconic acid and 4-O-\u03b2-D-galactopyranosyl-D-glucono-1,5-lactone. Content 98.0 per cent to 102.0 per cent (anhydrous substance). CHARACTERS Appearance White or almost white powder. Solubility Freely soluble in water, slightly soluble in…<\/p>\n","protected":false},"author":2,"featured_media":16709,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-16423","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/16423","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=16423"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/16423\/revisions"}],"predecessor-version":[{"id":16426,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/16423\/revisions\/16426"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/16709"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=16423"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=16423"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=16423"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}DEFINITION<\/h2>\n
Content<\/h3>\n
CHARACTERS<\/h2>\n
Appearance<\/h3>\n
Solubility<\/h3>\n
mp<\/h3>\n
IDENTIFICATION<\/h2>\n
TESTS<\/h2>\n
Appearance of solution<\/h3>\n
Specific optical rotation (2.2.7)<\/h4>\n
Reducing sugars<\/h3>\n
Water (2.5.12)<\/h4>\n
Total ash (2.4.16)<\/h4>\n
ASSAY<\/h2>\n