{"id":15420,"date":"2025-10-17T11:51:04","date_gmt":"2025-10-17T04:51:04","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=15420"},"modified":"2025-10-17T14:03:21","modified_gmt":"2025-10-17T07:03:21","slug":"glycerol-dibehenate","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/glycerol-dibehenate\/","title":{"rendered":"Glycerol Dibehenate"},"content":{"rendered":"<p><em>(Ph. Eur. monograph 1427)<\/em><\/p>\n<p><strong>Action and use<\/strong><\/p>\n<p>Excipient.<\/p>\n<h2>DEFINITION<\/h2>\n<p>Mixture of diacylglycerols, mainly dibehenoylglycerol, together with variable quantities of mono- and triacylglycerols, obtained by esterification of Glycerol (0496) with behenic (docosanoic) acid.<\/p>\n<h3>Content<\/h3>\n<p>\u2014 monoacylglycerols: 15.0 per cent to 23.0 per cent;<\/p>\n<p>\u2014 diacylglycerols: 40.0 per cent to 60.0 per cent;<\/p>\n<p>\u2014 triacylglycerols: 21.0 per cent to 35.0 per cent.<\/p>\n<h2>CHARACTERS<\/h2>\n<h3>Appearance<\/h3>\n<p>Hard, waxy mass, or powder or white or almost white, unctuous flakes.<\/p>\n<h3>Solubility<\/h3>\n<p>Practically insoluble in water, soluble in methylene chloride, partly soluble in hot ethanol (96 per cent).<\/p>\n<h2>IDENTIFICATION<\/h2>\n<p>A. Melting point (2.2.14): 65 \u00b0C to 77 \u00b0C.<\/p>\n<p>B. Composition of fatty acids (see Tests).<\/p>\n<p>C. It complies with the assay (content of diacylglycerols).<\/p>\n<h2>TESTS<\/h2>\n<h3>Acid value (2.5.1)<\/h3>\n<p>Maximum 4.0, determined on 1.0 g using a mixture of equal volumes of ethanol (96 per cent) R and toluene R as solvent and with gentle heating.<br \/>\nIodine value (2.5.4, Method A)<\/p>\n<p>Maximum 3.0.<\/p>\n<h3>Saponification value (2.5.6)<\/h3>\n<p>145 to 165.<\/p>\n<p>Carry out the titration with heating.<\/p>\n<h3>Free glycerol<\/h3>\n<p>Maximum 1.0 per cent, determined as described under Assay.<\/p>\n<h3>Composition of fatty acids<\/h3>\n<p>Gas chromatography (2.4.22, Method C) with the following modifications. Raise the temperature of the column to 240 \u00b0C and use the mixture of calibrating substances in Table 2.4.22.-3.<\/p>\n<p>Composition of the fatty acid fraction of the substance:<\/p>\n<p>\u2014 palmitic acid: maximum 3.0 per cent;<\/p>\n<p>\u2014 stearic acid: maximum 5.0 per cent;<\/p>\n<p>\u2014 arachidic acid: maximum 10.0 per cent;<\/p>\n<p>\u2014 behenic acid: minimum 83.0 per cent;<\/p>\n<p>\u2014 erucic acid: maximum 3.0 per cent;<\/p>\n<p>\u2014 lignoceric acid: maximum 3.0 per cent.<\/p>\n<h3>Water (2.5.12)<\/h3>\n<p>Maximum 1.0 per cent, determined on 1.00 g. Use pyridine R as the solvent.<\/p>\n<h3>Total ash (2.4.16)<\/h3>\n<p>Maximum 0.1 per cent, determined on 1.00 g.<\/p>\n<h2>ASSAY<\/h2>\n<p>Size-exclusion chromatography (2.2.30).<\/p>\n<p>Stock solution: Place 0.100 g of glycerol R in a flask and dilute to 25.0 mL with tetrahydrofuran R.<\/p>\n<p>Test solution: In a 15 mL flask, weigh 0.200 g (m) of the substance to be examined and add 5.0 mL of tetrahydrofuran R.<\/p>\n<p>Heat gently, at about 35 \u00b0C, and shake to dissolve. Reweigh the flask and calculate the total mass of solvent and substance (M); use immediately.<\/p>\n<p>Reference solutions: Into four 15 mL flasks, introduce respectively 0.25 mL, 0.5 mL, 1.0 mL and 2.5 mL of the stock solution and add 5.0 mL of tetrahydrofuran R. Weigh each flask and calculate the concentration of glycerol in milligrams per gram of each reference solution.<\/p>\n<p>Column:<\/p>\n<p>\u2014 size: l = 0.6 m, \u00d8 = 7 mm;<\/p>\n<p>\u2014 stationary phase: styrene-divinylbenzene copolymer R (5 \u03bcm) with a pore size of 10 nm.<\/p>\n<p>Mobile phase: tetrahydrofuran R.<\/p>\n<p>Flow rate: 1 mL\/min.<\/p>\n<p>Detection: Differential refractive index.<\/p>\n<p>Injection: 40 \u03bcL; when injecting the test solution, maintain the flask at about 35 \u00b0C to avoid precipitation.<\/p>\n<p>Relative retention: With reference to glycerol (retention time = about 15 min): triacylglycerols = about 0.73; diacylglycerols = about 0.76; monoacylglycerols = about 0.82.<\/p>\n<p>Calculations:<\/p>\n<p>\u2014 free glycerol: from the calibration curve obtained with the reference solutions, determine the concentration (C) in milligrams per gram in the test solution and calculate the percentage content (A) in the substance to be examined using the following expression:<\/p>\n<p style=\"text-align: center;\">(C x M)\/ (m x 10)<\/p>\n<p>\u2014 free fatty acids: calculate the percentage content of free fatty acids (D) using the following expression:<\/p>\n<p style=\"text-align: center;\">(I<sub>A<\/sub> x 340)\/ 561.1<\/p>\n<p>I<sub>A<\/sub> = acid value;<\/p>\n<p>\u2014 monoacylglycerols: calculate the percentage content of monoacylglycerols using the following expression:<\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-15463\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/10\/2-218.jpg\" alt=\"Glycerol Dibehenate \" width=\"250\" height=\"100\" \/><\/p>\n<p>A = percentage content of free glycerol (see Tests);<\/p>\n<p>B = percentage content of water (see Tests);<\/p>\n<p>D = percentage content of free fatty acids;<\/p>\n<p>X = area of the peak due to monoacylglycerols;<\/p>\n<p>Y = area of the peak due to diacylglycerols;<\/p>\n<p>Z = area of the peak due to triacylglycerols;<\/p>\n<p>\u2014 diacylglycerols: calculate the percentage content of diacylglycerols using the following expression:<\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-15464\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/10\/3-203.jpg\" alt=\"Glycerol Dibehenate \" width=\"250\" height=\"100\" \/><\/p>\n<p>\u2014 triacylglycerols: calculate the percentage content of triacylglycerols using the following expression:<\/p>\n<p style=\"text-align: center;\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-full wp-image-15465\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/10\/4-185.jpg\" alt=\"Glycerol Dibehenate \" width=\"250\" height=\"100\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<p>(Ph. Eur. monograph 1427) Action and use Excipient. DEFINITION Mixture of diacylglycerols, mainly dibehenoylglycerol, together with variable quantities of mono- and triacylglycerols, obtained by esterification of Glycerol (0496) with behenic (docosanoic) acid. Content \u2014 monoacylglycerols: 15.0 per cent to 23.0 per cent; \u2014 diacylglycerols: 40.0 per cent to 60.0 per cent; \u2014 triacylglycerols: 21.0 per&#8230;<\/p>\n","protected":false},"author":4,"featured_media":15422,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-15420","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/15420","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=15420"}],"version-history":[{"count":4,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/15420\/revisions"}],"predecessor-version":[{"id":15466,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/15420\/revisions\/15466"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/15422"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=15420"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=15420"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=15420"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}