{"id":1273,"date":"2025-09-18T11:44:17","date_gmt":"2025-09-18T04:44:17","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=1273"},"modified":"2025-10-02T17:09:08","modified_gmt":"2025-10-02T10:09:08","slug":"adipic-acid","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/adipic-acid\/","title":{"rendered":"Adipic Acid"},"content":{"rendered":"

(Ph. Eur. monograph 1586)<\/p>\n

C6<\/sub>H10<\/sub>O4<\/sub>\u00a0 \u00a0 \u00a0 146.1\u00a0 \u00a0 \u00a0 124-04-9<\/p>\n

Action and use<\/strong><\/p>\n

Excipient.<\/p>\n

DEFINITION<\/h2>\n

Hexanedioic acid.<\/p>\n

Content<\/h3>\n

99.0 per cent to 101.0 per cent (dried substance).<\/p>\n

CHARACTERS<\/h2>\n

Appearance<\/h3>\n

White or almost white, crystalline powder.<\/p>\n

Solubility<\/h3>\n

Sparingly soluble in water, soluble in boiling water, freely soluble in ethanol (96 per cent) and in methanol, soluble in acetone.<\/p>\n

IDENTIFICATION<\/h2>\n

A. Melting point (2.2.14): 151 \u00b0C to 154 \u00b0C.<\/p>\n

B. Infrared absorption spectrophotometry (2.2.24).<\/p>\n

Comparison adipic acid CRS.<\/p>\n

TESTS<\/h2>\n

Solution S<\/h3>\n

Dissolve 5.0 g with heating in distilled water R and dilute to 50 mL with the same solvent. Allow to cool and to crystallise. Filter through a sintered-glass filter (40) (2.1.2). Wash the filter with distilled water R. Collect the filtrate and the washings until a volume of 50 mL is obtained.<\/p>\n

Appearance of solution<\/h3>\n

The solution is clear (2.2.1) and colourless (2.2.2, Method II).<\/p>\n

Dissolve 1.0 g in methanol R and dilute to 20 mL with the same solvent.<\/p>\n

Related substances<\/h3>\n

Liquid chromatography (2.2.29).<\/p>\n

Test solution Dissolve 0.20 g of the substance to be examined in the mobile phase and dilute to 10.0 mL with the mobile phase.<\/p>\n

Reference solution (a) Dissolve 20 mg of glutaric acid R in 1.0 mL of the test solution and dilute to 10.0 mL with the mobile phase.<\/p>\n

Reference solution (b) Dilute 1.0 mL of the test solution to 100.0 mL with the mobile phase, dilute 1.0 mL of the solution to 10.0 mL with the mobile phase.<\/p>\n

Column:<\/p>\n

\u2014 size: l = 0.125 m, \u00d8 = 4.0 mm,<\/p>\n

\u2014 stationary phase: spherical octadecylsilyl silica gel for chromatography R (5 \u03bcm) with a specific surface area of 350 m \/g and a pore size of 10 nm,<\/p>\n

\u2014 temperature: 30 \u00b0C.<\/p>\n

Mobile phase Mix 3 volumes of acetonitrile R and 97 volumes of a 24.5 g\/L solution of dilute phosphoric acid R.<\/p>\n

Flow rate 1 mL\/min.<\/p>\n

Detection Spectrophotometer at 209 nm.<\/p>\n

Injection 20 \u03bcL.<\/p>\n

Run time 3 times the retention time of adipic acid.<\/p>\n

System suitability Reference solution (a):<\/p>\n

\u2014 resolution: minimum 9.0 between the peaks due to glutaric acid and adipic acid.<\/p>\n

Limits:<\/p>\n

\u2014 any impurity: not more than the area of the principal peak in the chromatogram obtained with reference solution (b) (0.1 per cent),<\/p>\n

\u2014 total: not more than 5 times the area of the principal peak in the chromatogram obtained with reference solution (b) (0.5 per cent),<\/p>\n

\u2014 disregard limit: 0.5 times the area of the principal peak in the chromatogram obtained with reference solution (b) (0.05 per cent).<\/p>\n

Chlorides (2.4.4)<\/h3>\n

Maximum 200 ppm.<\/p>\n

Dilute 2.5 mL of solution S to 15 mL with water R.<\/p>\n

Nitrates<\/h3>\n

Maximum 30 ppm.<\/p>\n

To 1 mL of solution S add 2 mL of concentrated ammonia R, 0.5 mL of a 10 g\/L solution of manganese sulfate R, 1 mL of a 10 g\/L solution of sulfanilamide R and dilute to 20 mL with water R. Add 0.10 g of zinc powder R and cool in iced water for 30 min; shake from time to time. Filter and cool 10 mL of the filtrate in iced water. Add 2.5 mL of hydrochloric acid R1 and 1 mL of a 10 g\/L solution of naphthylethylenediamine dihydrochloride R. Allow to stand at room temperature. After 15 min the mixture is not more intensely coloured than a standard prepared at the same time and in the same manner, using 1.5 mL of nitrate standard solution (2 ppm NO3<\/sub>) R instead of 1 mL of solution S. The test is invalid if a blank solution prepared at the same time and in the same manner, using 1 mL of water R instead of 1 mL of solution S, is more intensely coloured than a 2 mg\/L solution of potassium permanganate R.<\/p>\n

Sulfates (2.4.13)<\/h3>\n

Maximum 500 ppm.<\/p>\n

Dilute 3 mL of solution S to 15 mL with distilled water R.<\/p>\n

Iron (2.4.9)<\/h3>\n

Maximum 10 ppm, determined on solution S.<\/p>\n

Loss on drying (2.2.32)<\/h4>\n

Maximum 0.2 per cent, determined on 1.000 g by drying in an oven at 105 \u00b0C.<\/p>\n

Sulfated ash (2.4.14)<\/h4>\n

Maximum 0.1 per cent.<\/p>\n

Melt 1.0 g completely over a gas burner, then ignite the melted substance with the burner. After ignition, lower or remove the flame in order to prevent the substance from boiling and keep it burning until completely carbonised. Carry out the test for sulfated ash using the residue.<\/p>\n

ASSAY<\/h2>\n

Dissolve 60.0 mg in 50 mL of water R. Add 0.2 mL of phenolphthalein solution R and titrate with 0.1 M sodium hydroxide. 1 mL of 0.1 M sodium hydroxide is equivalent to 7.31 mg of C6<\/sub>H10<\/sub>O4<\/sub>.<\/p>\n

IMPURITIES<\/h2>\n

\"Adipic<\/p>\n

A. pentanedioic acid (glutaric acid),<\/p>\n

\"Adipic<\/p>\n

B. butanedioic acid (succinic acid),<\/p>\n

\"Adipic<\/p>\n

C. heptanedioic acid (pimelic acid).<\/p>\n","protected":false},"excerpt":{"rendered":"

(Ph. Eur. monograph 1586) C6H10O4\u00a0 \u00a0 \u00a0 146.1\u00a0 \u00a0 \u00a0 124-04-9 Action and use Excipient. DEFINITION Hexanedioic acid. Content 99.0 per cent to 101.0 per cent (dried substance). CHARACTERS Appearance White or almost white, crystalline powder. Solubility Sparingly soluble in water, soluble in boiling water, freely soluble in ethanol (96 per cent) and in methanol,…<\/p>\n","protected":false},"author":2,"featured_media":1278,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-1273","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/1273","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=1273"}],"version-history":[{"count":5,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/1273\/revisions"}],"predecessor-version":[{"id":6907,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/1273\/revisions\/6907"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/1278"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=1273"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=1273"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=1273"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}