{"id":1199,"date":"2025-09-18T15:07:45","date_gmt":"2025-09-18T08:07:45","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=1199"},"modified":"2025-10-01T15:35:27","modified_gmt":"2025-10-01T08:35:27","slug":"acetylene-intermix-in-nitrogen","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/acetylene-intermix-in-nitrogen\/","title":{"rendered":"Acetylene Intermix (1 per cent) in Nitrogen"},"content":{"rendered":"<p><em>(Ph. Eur. monograph 2903)<\/em><\/p>\n<h2>DEFINITION<\/h2>\n<p>A mixture containing 1 per cent V\/V of acetylene in Low-oxygen nitrogen (1685).<\/p>\n<h3>Content<\/h3>\n<p>0.95 per cent V\/V to 1.05 per cent V\/V of acetylene (C<sub>2<\/sub>H<sub>2<\/sub>) in nitrogen (N<sub>2<\/sub>).<\/p>\n<p>This monograph applies to acetylene intermix (1 per cent) in nitrogen used in the preparation of lung function test gas mixtures for medicinal use.<\/p>\n<h2>PRODUCTION<\/h2>\n<p>The acetylene used in the manufacturing process is produced by hydrolysis of calcium carbide.<\/p>\n<p>Prior to using the gas in the manufacturing process, the acetylene may be passed through an activated charcoal filter.<\/p>\n<p>The acetylene is stored in cylinders, which may be filled with a porous mass with acetone the only solvent permitted.<\/p>\n<h2>CHARACTERS<\/h2>\n<h3>Appearance<\/h3>\n<p>Colourless gas.<\/p>\n<h2>IDENTIFICATION<\/h2>\n<p>A. Examine the chromatograms obtained in the assay.<\/p>\n<p><em>Results: <\/em>The peak due to acetylene in the chromatogram obtained with the gas to be examined is similar in retention time and size to the peak due to acetylene in the chromatogram obtained with the reference gas.<\/p>\n<p>B. Gas chromatography (2.2.28).<\/p>\n<p><em>Gas to be examined:<\/em>\u00a0The substance to be examined.<\/p>\n<p><em>Reference gas:<\/em>\u00a0Nitrogen R1.<\/p>\n<p><em>Column:<\/em><\/p>\n<p><em>\u2014 material:<\/em> stainless steel;<\/p>\n<p><em>\u2014 size:<\/em> l = 2 m, \u00d8 = 2 mm;<\/p>\n<p><em>\u2014 stationary phase:<\/em> molecular sieve for chromatography R (0.5 nm).<\/p>\n<p><em>Carrier gas:<\/em>\u00a0helium for chromatography R.<\/p>\n<p><em>Flow rate:<\/em>\u00a020 mL\/min.<\/p>\n<p><em>Temperature:<\/em><\/p>\n<p><em>\u2014 column:<\/em> 80 \u00b0C;<\/p>\n<p><em>\u2014 detector:<\/em> 130 \u00b0C.<\/p>\n<p><em>Detection\u00a0<\/em> Thermal conductivity.<\/p>\n<p><em>Injection\u00a0<\/em> 10 \u03bcL.<\/p>\n<p><em>Retention time:<\/em>\u00a0Nitrogen = about 2 min.<\/p>\n<p><em>Results:<\/em>\u00a0The principal peak in the chromatogram obtained with the gas to be examined is similar in retention time to the principal peak in the chromatogram obtained with the reference gas.<\/p>\n<h2>TESTS<\/h2>\n<h3>Acetone<\/h3>\n<p>Gas chromatography (2.2.28).<\/p>\n<p><em>Gas to be examined:<\/em>\u00a0The substance to be examined.<\/p>\n<p><em>Reference gas:<\/em>\u00a0Mixture containing 250 ppm V\/V of acetone R in nitrogen R.<\/p>\n<p><em>Column:<\/em><\/p>\n<p><em>\u2014 material:<\/em> fused silica;<\/p>\n<p><em>\u2014 size:<\/em> l = 10 m, \u00d8 = 0.53 mm;<\/p>\n<p><em>\u2014 stationary phase:<\/em> polyorganosiloxane for oxygen-containing compounds R (film thickness 10 \u03bcm).<\/p>\n<p><em>Carrier gas:<\/em>\u00a0helium for chromatography R.<\/p>\n<p><em>Flow rate:<\/em>\u00a050 mL\/min.<\/p>\n<p><em>Temperature:<\/em><\/p>\n<p><em>\u2014 column:<\/em> 200 \u00b0C;<\/p>\n<p><em>\u2014 injection port:<\/em> 240 \u00b0C;<\/p>\n<p><em>\u2014 detector:<\/em> 250 \u00b0C.<\/p>\n<p><em>Detection\u00a0<\/em> Flame ionisation.<\/p>\n<p><em>Injection\u00a0<\/em> 25 \u03bcL.<\/p>\n<p><em>Retention time:<\/em>\u00a0Acetone = about 1 min.<\/p>\n<p><em>Calculation of percentage content:<\/em><\/p>\n<p>\u2014 use the concentration of acetone in the reference gas.<\/p>\n<p><em>Limit:<\/em><\/p>\n<p><em>\u2014 acetone:<\/em> maximum 250 ppm V\/V.<\/p>\n<h3>Arsine<\/h3>\n<p>Maximum 0.25 ppm V\/V, determined using an arsine detector tube (2.1.6).<\/p>\n<h3>Phosphine<\/h3>\n<p>Maximum 0.2 ppm V\/V, determined using a phosphine detector tube (2.1.6).<\/p>\n<h3>Hydrogen sulfide<\/h3>\n<p>Maximum 0.2 ppm V\/V, determined using a hydrogen sulfide detector tube (2.1.6).<\/p>\n<h3>Water (2.5.28)<\/h3>\n<p>Maximum 10 ppm V\/V.<\/p>\n<h2>ASSAY<\/h2>\n<p>Gas chromatography (2.2.28).<\/p>\n<p><em>Gas to be examined:<\/em>\u00a0The substance to be examined.<\/p>\n<p><em>Reference gas:<\/em>\u00a0Mixture containing 1.0 per cent V\/V of acetylene R in nitrogen R1.<\/p>\n<p><em>Column:<\/em><\/p>\n<p><em>\u2014 material:<\/em> stainless steel;<\/p>\n<p><em>\u2014 size:<\/em> l = 2 m, \u00d8 = 2 mm;<\/p>\n<p><em>\u2014 stationary phase:<\/em> 3 per cent squalane R on alumina.<\/p>\n<p><em>Carrier gas:<\/em>\u00a0helium for chromatography R.<\/p>\n<p><em>Flow rate:<\/em>\u00a020 mL\/min.<\/p>\n<p><em>Temperature:<\/em><\/p>\n<p><em>\u2014 column:<\/em> 100 \u00b0C;<\/p>\n<p><em>\u2014 detector:<\/em> 250 \u00b0C.<\/p>\n<p><em>Detection:<\/em>\u00a0Flame ionisation.<\/p>\n<p><em>Injection:<\/em>\u00a0100 \u03bcL.<\/p>\n<p><em>Retention time:<\/em>\u00a0Acetylene = about 6 min.<\/p>\n<p>Calculate the percentage content of C<sub>2<\/sub>H<sub>2<\/sub>.<\/p>\n<h2>STORAGE<\/h2>\n<p>As a compressed gas, in appropriate high-pressure cylinders complying with the legal regulations.<\/p>\n<h2>LABELLING<\/h2>\n<p>The label states the nominal content, in per cent V\/V, of acetylene in nitrogen.<\/p>\n<h2>IMPURITIES<\/h2>\n<p>Specified impurities A, B, C, D, E.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7200\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/3-173-300x163.jpg\" alt=\"Acetylene Intermix in Nitrogen\" width=\"300\" height=\"163\" srcset=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/3-173-300x163.jpg 300w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/3-173-1024x555.jpg 1024w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/3-173-768x416.jpg 768w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/3-173.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>A. propan-2-one (acetone),<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7201\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/4-156-300x163.jpg\" alt=\"Acetylene Intermix in Nitrogen\" width=\"300\" height=\"163\" srcset=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/4-156-300x163.jpg 300w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/4-156-1024x555.jpg 1024w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/4-156-768x416.jpg 768w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/4-156.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><br \/>\nB. arsane (arsine),<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7202\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/5-136-300x163.jpg\" alt=\"Acetylene Intermix in Nitrogen\" width=\"300\" height=\"163\" srcset=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/5-136-300x163.jpg 300w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/5-136-1024x555.jpg 1024w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/5-136-768x416.jpg 768w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/5-136.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><br \/>\nC. phosphane (phosphine),<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7203\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/6-119-300x163.jpg\" alt=\"Acetylene Intermix in Nitrogen\" width=\"300\" height=\"163\" srcset=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/6-119-300x163.jpg 300w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/6-119-1024x555.jpg 1024w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/6-119-768x416.jpg 768w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/6-119.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><br \/>\nD. hydrogen sulfide,<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-7204\" src=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/7-101-300x163.jpg\" alt=\"Acetylene Intermix in Nitrogen\" width=\"300\" height=\"163\" srcset=\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/7-101-300x163.jpg 300w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/7-101-1024x555.jpg 1024w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/7-101-768x416.jpg 768w, https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/09\/7-101.jpg 1200w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><br \/>\nE. water.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>(Ph. Eur. monograph 2903) DEFINITION A mixture containing 1 per cent V\/V of acetylene in Low-oxygen nitrogen (1685). Content 0.95 per cent V\/V to 1.05 per cent V\/V of acetylene (C2H2) in nitrogen (N2). This monograph applies to acetylene intermix (1 per cent) in nitrogen used in the preparation of lung function test gas mixtures&#8230;<\/p>\n","protected":false},"author":4,"featured_media":1866,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-1199","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/1199","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=1199"}],"version-history":[{"count":5,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/1199\/revisions"}],"predecessor-version":[{"id":7206,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/1199\/revisions\/7206"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/1866"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=1199"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=1199"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=1199"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}