![\"Farmed](\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/10\/1-112-300x163.jpg\")
<\/p>\n(Ph. Eur. monograph 1910)<\/p>\n
Purified fatty oil obtained from fresh farmed Salmo salar. The positional distribution (\u03b2(2)-acyl) is 60-70 per
\ncent for cervonic (docosahexaenoic) acid (C22:6 n-3; DHA), 25-35 per cent for timnodonic
\n(eicosapentaenoic) acid (C20:5 n-3; EPA) and 40-55 per cent for moroctic acid (C18:4 n-3).<\/p>\n
\u2014 sum of the contents of EPA and DHA (expressed as triglycerides): 10.0 per cent to 28.0 per cent.
\nA suitable antioxidant may be added.<\/p>\n
The fish shall only be given feed with a composition that is in accordance with the relevant European Union
\nor other applicable regulations.<\/p>\n
The content of dioxins and dioxin-like PCBs (polychlorinated biphenyls) is controlled using methods and
\nlimits in accordance with the requirements set in the European Union or other applicable regulations.<\/p>\n
The oil is produced by mechanical expression of fresh raw materials, either from the whole fish, or fish
\nwhere the fillets have been removed, at a temperature not exceeding 100 \u00b0C, and without using solvents.
\nAfter centrifugation, solid substances may be removed from the oil by cooling and filtering (winterisation).<\/p>\n
Pale pink liquid.<\/p>\n
Farmed Salmon Oil<\/p>\n
Practically insoluble in water, very soluble in acetone and in heptane, slightly soluble in anhydrous ethanol.<\/p>\n
Examine the C NMR spectra obtained in the assay for positional distribution (\u03b2(2)-acyl) of fatty acids. The
\nspectra contain peaks between 172 ppm and 173 ppm with shifts similar to those in the type spectrum
\n(Figure 1910.-2). The oil to be examined complies with the limits of this assay.<\/p>\n
Minimum 0.10, measured at the absorption maximum between 470 nm and 480 nm.<\/p>\n
Dissolve 5.0 mL in 5.0 mL of trimethylpentane R.<\/p>\n
Maximum 2.0.<\/p>\n
Maximum 10.0.<\/p>\n
Maximum 5.0.<\/p>\n
Maximum 1.5 per cent, determined on 5.0 g.<\/p>\n
Maximum 11.0 per cent.<\/p>\n
Identify the peak due to linoleic acid using the chromatogram in Figure 1910.-1. Determine the percentage content by normalisation.<\/p>\n
Nuclear magnetic resonance spectrometry (2.2.33).<\/p>\n
Apparatus High resolution FT-NMR spectrometer operating at minimum 300 MHz.<\/p>\n
Test solution Dissolve 190-210 mg of fresh salmon oil in 500 \u03bcL of deuterated chloroform R. Prepare at
\nleast 3 samples and examine within 3 days.<\/p>\n
Acquisition of C NMR spectra The following parameters may be used:<\/p>\n
\u2014 sweep width: 200 ppm (-5 to 195 ppm);<\/p>\n
\u2014 irradiation frequency offset: 95 ppm;<\/p>\n
\u2014 time domain: 64 K;<\/p>\n
\u2014 pulse delay: 2 s;<\/p>\n
\u2014 pulse program: zgig 30 (inverse gated, 30\u00b0 excitation pulse);<\/p>\n
\u2014 dummy scans: 4;<\/p>\n
\u2014 number of scans: 4096.<\/p>\n
Processing and plotting The following parameters may be used:<\/p>\n
\u2014 size: 64 K (zero-filling);<\/p>\n
\u2014 window multiplication: exponential;<\/p>\n
\u2014 Lorentzian broadening factor: 0.2 Hz.<\/p>\n
Use the CDCl3 signal for shift referencing. The shift of the central peak of the 1:1:1 triplet is set to 77.16 ppm.<\/p>\n
Plot the spectral region \u03b4 171.5-173.5 ppm. Compare the spectrum with the reference spectrum in Figure 1910.-2. The shift values lie within the ranges given in Table 1910.-1.<\/p>\n
<\/p>\n
1. C14:0 5. C18:0 9. C18:3 n-3 13. C20:4 n-3 17. C22:5 n-6<\/p>\n
2. C16:0 6. C18:1 n-9 10. C18:4 n-3 14. EPA 18. C22:5 n-3<\/p>\n
3. C16:1 n-7 7. C18:1 n-7 11. C20:1 n-9 15. C22:1 n-11 19. DHA<\/p>\n
4. C16:4 n-1 8. C18:2 n-6 12. C20:4 n-6 16. C21:5 n-3<\/p>\n
Figure 1910.-1. \u2013 Chromatogram for the composition of fatty acids in farmed salmon oil<\/p>\n
Table 1910.-1. \u2013 Shift values<\/p>\n
| Signal<\/td>\n | Shift range \n(ppm)<\/td>\n<\/tr>\n |
| \u03b2 DHA<\/td>\n | 172.05 – 172.09<\/td>\n<\/tr>\n |
| \u03b1 DHA<\/td>\n | 172.43 – 172.47<\/td>\n<\/tr>\n |
| \u03b2 EPA<\/td>\n | 172.52 – 172.56<\/td>\n<\/tr>\n |
| \u03b1 EPA<\/td>\n | 172.90 – 172.94<\/td>\n<\/tr>\n |
| \u03b2 C18:4<\/td>\n | 172.56 – 172.60<\/td>\n<\/tr>\n |
| \u03b1 C18:4<\/td>\n | 172.95 – 172.99<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n![\"Farmed](\"https:\/\/nhathuocngocanh.com\/bp\/wp-content\/uploads\/2025\/10\/2-111-300x163.jpg\") 1. \u03b1 C18:4 2. \u03b1 EPA 3. \u03b2 C18:4 4. \u03b2 EPA 5. \u03b1 DHA 6. \u03b2 DHA<\/p>\n Figure 1910.-2. \u2013 C NMR spectrum: carbonyl region of farmed salmon oil<\/p>\n System suitability:<\/p>\n \u2014 signal-to-noise ratio: minimum 5 for the smallest relevant peak corresponding to \u03b1 C18:4 signal (in \u2014 peak width at half-height maximum 0.02 ppm for the central CDCl3<\/sub> signal (at \u03b4 77.16 ppm).<\/p>\n Calculation of positional distribution (\u03b2(2)-acyl) Use the following expression:<\/p>\n \u03b2\/\u03b1+\u03b2 \u00d7 100<\/p>\n \u03b1 = peak area of the corresponding \u03b1-carbonyl peak;<\/p>\n \u03b2 = peak area of \u03b2-carbonyl peak from C22:6 n-3, C20:5 n-3 or C18:4 n-3, respectively.<\/p>\n Limits:<\/p>\n \u2014 cervonic (docosahexaenoic) acid (C22:6 n-3; DHA): 60 per cent to 70 per cent;<\/p>\n \u2014 timnodonic (eicosapentaenoic) acid (C20:5 n-3; EPA): 25 per cent to 35 per cent;<\/p>\n \u2014 moroctic acid (C18:4 n-3): 40 per cent to 55 per cent.<\/p>\n See Figure 1910.-1.<\/p>\n In an airtight, well-filled container, protected from light, under inert gas.<\/p>\n The label states:<\/p>\n \u2014 the concentration of EPA and DHA, expressed as triglycerides.<\/p>\n","protected":false},"excerpt":{"rendered":" (Ph. Eur. monograph 1910) DEFINITION Purified fatty oil obtained from fresh farmed Salmo salar. The positional distribution (\u03b2(2)-acyl) is 60-70 per cent for cervonic (docosahexaenoic) acid (C22:6 n-3; DHA), 25-35 per cent for timnodonic (eicosapentaenoic) acid (C20:5 n-3; EPA) and 40-55 per cent for moroctic acid (C18:4 n-3). Content \u2014 sum of the contents of…<\/p>\n","protected":false},"author":3,"featured_media":11966,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-11590","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/11590","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=11590"}],"version-history":[{"count":3,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/11590\/revisions"}],"predecessor-version":[{"id":11967,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/11590\/revisions\/11967"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/11966"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=11590"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=11590"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=11590"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}} |