{"id":11227,"date":"2025-10-09T11:16:01","date_gmt":"2025-10-09T04:16:01","guid":{"rendered":"https:\/\/nhathuocngocanh.com\/bp\/?p=11227"},"modified":"2025-10-09T11:16:01","modified_gmt":"2025-10-09T04:16:01","slug":"ethyl-hydroxybenzoate","status":"publish","type":"post","link":"https:\/\/nhathuocngocanh.com\/bp\/ethyl-hydroxybenzoate\/","title":{"rendered":"Ethyl Hydroxybenzoate"},"content":{"rendered":"

Ethylparaben<\/p>\n

(Ethyl Parahydroxybenzoate, Ph. Eur. monograph 0900)<\/p>\n

C9<\/sub>H10<\/sub>O3<\/sub>\u00a0 \u00a0166.2\u00a0 \u00a0120-47-8<\/p>\n

DEFINITION<\/h2>\n

Ethyl 4-hydroxybenzoate.<\/p>\n

Content<\/h3>\n

98.0 per cent to 102.0 per cent.<\/p>\n

\u2666 CHARACTERS<\/h2>\n

Appearance<\/h3>\n

White or almost white, crystalline powder or colourless crystals.<\/p>\n

Solubility<\/h3>\n

Very slightly soluble in water, freely soluble in ethanol (96 per cent) and in methanol.\u2666<\/p>\n

IDENTIFICATION<\/h2>\n

First identification: A, B.<\/p>\n

\u2662 Second identification: A, C.\u2662<\/p>\n

A. Melting point (2.2.14): 115 \u00b0C to 118 \u00b0C.<\/p>\n

B. Infrared absorption spectrophotometry (2.2.24).<\/p>\n

Comparison ethyl parahydroxybenzoate CRS.<\/p>\n

\u2662 C. Thin-layer chromatography (2.2.27).<\/p>\n

Test solution (a): Dissolve 0.10 g of the substance to be examined in acetone R and dilute to 10 mL with the same solvent.<\/p>\n

Test solution (b): Dilute 1 mL of test solution (a) to 10 mL with acetone R.<\/p>\n

Reference solution (a): Dissolve 10 mg of ethyl parahydroxybenzoate CRS in acetone R and dilute to 10 mL with the same solvent.<\/p>\n

Reference solution (b): Dissolve 10 mg of methyl parahydroxybenzoate R in 1 mL of test solution (a) and dilute to 10 mL with acetone R.<\/p>\n

Plate TLC octadecylsilyl silica gel F254 plate R.<\/p>\n

Mobile phase glacial acetic acid R, water R, methanol R (1:30:70 V\/V\/V).<\/p>\n

Application 2 \u03bcL of test solution (b) and reference solutions (a) and (b).<\/p>\n

Development Over 2\/3 of the plate.<\/p>\n

Drying In air.<\/p>\n

Detection Examine in ultraviolet light at 254 nm.<\/p>\n

System suitability Reference solution (b):<\/p>\n

\u2014 the chromatogram shows 2 clearly separated principal spots.<\/p>\n

Results The principal spot in the chromatogram obtained with test solution (b) is similar in position and size to the principal spot in the chromatogram obtained with reference solution (a).\u2662<\/p>\n

TESTS<\/h2>\n

Solution S<\/h3>\n

Dissolve 1.0 g in ethanol (96 per cent) R and dilute to 10 mL with the same solvent.<\/p>\n

Appearance of solution<\/h3>\n

Solution S is clear (2.2.1) and not more intensely coloured than reference solution BY6 (2.2.2, Method II).<\/p>\n

Acidity<\/h3>\n

To 2 mL of solution S add 3 mL of ethanol (96 per cent) R, 5 mL of carbon dioxide-free water R and 0.1 mL of bromocresol green solution R. Not more than 0.1 mL of 0.1 M sodium hydroxide is required to change the colour of the indicator to blue.<\/p>\n

Related substances<\/h3>\n

Liquid chromatography (2.2.29).<\/p>\n

Test solution Dissolve 50.0 mg of the substance to be examined in 2.5 mL of methanol R and dilute to 50.0 mL with the mobile phase. Dilute 10.0 mL of this solution to 100.0 mL with the mobile phase.<\/p>\n

Reference solution (a): Dissolve 5 mg of 4-hydroxybenzoic acid R (impurity A), 5 mg of methyl parahydroxybenzoate R (impurity B) and 5 mg of the substance to be examined in the mobile phase and dilute to 100.0 mL with the mobile phase.<\/p>\n

Dilute 1.0 mL of this solution to 10.0 mL with the mobile phase.<\/p>\n

Reference solution (b): Dissolve 50.0 mg of ethyl parahydroxybenzoate CRS in 2.5 mL of methanol R and dilute to 50.0 mL with the mobile phase. Dilute 10.0 mL of this solution to 100.0 mL with the mobile phase.<\/p>\n

Reference solution (c): Dilute 1.0 mL of the test solution to 20.0 mL with the mobile phase. Dilute 1.0 mL of this solution to 10.0 mL with the mobile phase.<\/p>\n

Column:<\/p>\n

\u2014 size: l = 0.15 m, \u00d8 = 4.6 mm;<\/p>\n

\u2014 stationary phase: end-capped octadecylsilyl silica gel for chromatography R (5 \u03bcm).<\/p>\n

Mobile phase 6.8 g\/L solution of potassium dihydrogen phosphate R, methanol R (35:65 V\/V).<\/p>\n

Flow rate 1.3 mL\/min.<\/p>\n

Detection Spectrophotometer at 272 nm.<\/p>\n

Injection 10 \u03bcL of the test solution and reference solutions (a) and (c).<\/p>\n

Run time 4 times the retention time of ethyl parahydroxybenzoate.<\/p>\n

Relative retention With reference to ethyl parahydroxybenzoate (retention time = about 3.0 min): impurity A = about 0.5; impurity B = about 0.8.<\/p>\n

System suitability Reference solution (a):<\/p>\n

\u2014 resolution: minimum 2.0 between the peaks due to impurity B and ethyl parahydroxybenzoate.<\/p>\n

Limits:<\/p>\n

\u2014 correction factor: for the calculation of content, multiply the peak area of impurity A by 1.4;<\/p>\n

\u2014 impurity A: not more than the area of the principal peak in the chromatogram obtained with reference solution (c) (0.5 per cent);<\/p>\n

\u2014 unspecified impurities: for each impurity, not more than the area of the principal peak in the chromatogram obtained with reference solution (c) (0.5 per cent);<\/p>\n

\u2014 total: not more than twice the area of the principal peak in the chromatogram obtained with reference solution (c) (1.0 per cent);<\/p>\n

\u2014 disregard limit: 0.2 times the area of the principal peak in the chromatogram obtained with reference solution (c) (0.1 per cent).<\/p>\n

Sulfated ash (2.4.14)<\/h4>\n

Maximum 0.1 per cent, determined on 1.0 g.<\/p>\n

ASSAY<\/h2>\n

Liquid chromatography (2.2.29) as described in the test for related substances with the following modification.<\/p>\n

Injection Test solution and reference solution (b).<\/p>\n

Calculate the percentage content of C9<\/sub>H10<\/sub>O3<\/sub>\u00a0 from the declared content of ethyl parahydroxybenzoate CRS.<\/p>\n

IMPURITIES<\/h2>\n

Specified impurities A.<\/p>\n

Other detectable impurities (the following substances would, if present at a sufficient level, be detected by one or other of the tests in the monograph. They are limited by the general acceptance criterion for other\/unspecified impurities and\/or by the general monograph Substances for pharmaceutical use (2034). It is therefore not necessary to identify these impurities for demonstration of compliance. See also 5.10. Control of impurities in substances for pharmaceutical use) B, C, D.<\/p>\n

\"Ethyl<\/p>\n

A. 4-hydroxybenzoic acid,<\/p>\n

\"Ethyl<\/p>\n

B. methyl 4-hydroxybenzoate (methyl parahydroxybenzoate),<\/p>\n

\"Ethyl<\/p>\n

C. propyl 4-hydroxybenzoate (propyl parahydroxybenzoate),<\/p>\n

\"Ethyl<\/p>\n

D. butyl 4-hydroxybenzoate (butyl parahydroxybenzoate).<\/p>\n","protected":false},"excerpt":{"rendered":"

Ethylparaben (Ethyl Parahydroxybenzoate, Ph. Eur. monograph 0900) C9H10O3\u00a0 \u00a0166.2\u00a0 \u00a0120-47-8 DEFINITION Ethyl 4-hydroxybenzoate. Content 98.0 per cent to 102.0 per cent. \u2666 CHARACTERS Appearance White or almost white, crystalline powder or colourless crystals. Solubility Very slightly soluble in water, freely soluble in ethanol (96 per cent) and in methanol.\u2666 IDENTIFICATION First identification: A, B. \u2662…<\/p>\n","protected":false},"author":3,"featured_media":11245,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"footnotes":""},"categories":[174],"tags":[],"class_list":["post-11227","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-medicinal-substances"],"acf":[],"_links":{"self":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/11227","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/comments?post=11227"}],"version-history":[{"count":2,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/11227\/revisions"}],"predecessor-version":[{"id":11247,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/posts\/11227\/revisions\/11247"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media\/11245"}],"wp:attachment":[{"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/media?parent=11227"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/categories?post=11227"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/nhathuocngocanh.com\/bp\/wp-json\/wp\/v2\/tags?post=11227"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}